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| Identification of a Tobacco (Nicotiana tobacum) PGPR Strain and Optimization for Its Fermentation Conditions by Response Surface Analysis Method |
| WU Xiang*, XIE Li-Yuan, TAN Hao, GAN Bing-Cheng, PENG Wei-Hong, HUANG Zhong-Qian |
| Soil and Fertilizer institute, Sichuan Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Southwestern Region Agromicrobiological Resource Utilization, Ministry of Agriculture/Key Laboratory of Agricultural Environment in Southwest Mountain Areas, Ministry of Agriculture, Chengdu 610066, China |
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Abstract The definite taxonomic classification and fermentation conditions of the functional bacterial could be more efficient for biofertilizer development. Plant growth-promoting rhizobacteria (PGPR) strain MT-002-B-12 is promising isolate for tobacco biofertilizer production as a potential supplementary microbe. The aim of this study is to identify its taxonomic classification, and further optimize the conditions in fermentation. The phylogeny of PGPR strain MT-002-B-12 was analyzed by phenotypic features, physiological-biochemical characteristics and 16S rDNA gene sequence alignments. The fermentation conditions of MT-002-B-12 to produce indole-3-acetic acid (IAA) were optimized by combining the single factor condition analysis and response surface analysis method. The result of classification showed that MT-002-B-12 was Klebsiella variicola. The fermentation experiments showed that the optimum fermentation conditions for MT-002-B-12 was culture volume 30% (V/V), initial pH 6.0, temperature 25 ℃, shaker speed 150 r/min, culture time 34 h; and the optimum content of nutrients in the medium was 1.11% sucrose, 1.36% peptone, 0.15‰ MgSO4·7H2O, 0.34‰ CaCl2·2H2O. The strain MT-002-B-12 enriched the species of growth promoting bacteria, and could be used as a strain resource for the development of tobacco biofertilizer.
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Received: 11 April 2019
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Corresponding Authors:
* qingshui028@126.com
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