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| Identification and Biological Characteristics of Pathogens in Potato Common Scab |
| LI Chi1, LIU Yan1, LIANG Yan1, XING Xing1, ZHANG Jian-Ping3, LI De-Zhou1, 2, *, ZHANG Xiao-Yu1, * |
1 College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010019, China;
2 Development Planning Office, Inner Mongolia Agricultural University, Hohhot 010018, China;
3 Institute of Plant Protection, Inner Mongolia Academy of Agriculture and Animal Husbandry, Hohhot 010031, China |
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Abstract Potato common scab by Streptomyces spp.is a serious disease occurring within the western growing region of Inner Mongolia and is a major obstacle to potato (Solanum tuberosum) production.In order to clarify the pathogens and biological characteristics of potato common scab in Wuchuan county, Inner Mongolia, the pathogens were isolated by dilution separation method, and one isolate PS1 was obtained.The pathogenicity was determined by potato chip method and potted inoculation, and the pathogenic genes were detected by specific primers.In results, The strain PS1 could colonize in potato chips and produce brown necrosis, and produce typical scab symptoms in potted potato tubers.The pathogenic genes txtAB, tomA and nec1 (necrosis-inducing protein) were detected.The morphological characteristics, physiological and biochemical characteristics and 16S rDNA sequence were analyzed to identify the pathogens, and the isolate was identified as Streptomyces galilaeus.The results of biological characteristics indicated that the oat liquid medium for mycelium growth was optimum.When cultivated 10 d the mycelium dry weight was maximum.The optimum pH was 7, and the optimum cultivation temperature was 32 ℃.The optimum single carbon and nitrogen source were glucose and L-methionine.In this study, the pathogen species and biological characteristics in potato common scab were clarified, which laid the foundation for further study of epidemic patterns an control measures.
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Received: 17 November 2018
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Corresponding Authors:
zxy2000@126.com;lzdb33@163.com
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