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| GB Content and Expression of BADH Genes in Cassava (Manihot esculenta) Under Drought and NaCl Stress |
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Abstract Abstract Betaine aldehyde dehydrogenase (BADH) is the key enzyme in the synthesis process of glycine betaine (GB). To explore the expression difference of BADH gene in different tissues of cassava (Manihot esculenta) and the expression under drought and salt stress, bioinformatics approaches were used to identify and characterize MeBADH genes. Using computational methods, these genes were localized on cassava genome chromosome and stress-responsive cis-elements within their 5' upstream regions were identified. Expression profiles of these genes in different tissues were detected by qRT-PCR, at the same time, the differential expression of MeBADH genes under PEG-6000 stress, salt stress and natural drought stress were investigated in cassava. The GB content in cassava was determined by ELISA. The results showed that MeBADHs genes expressed in all tissues (root, root tuber, stem, leaf). The expression in root tuber was significantly higher than that in root (P＜0.05). The expression of MeBADH1 and MeBADH2 in leaves, stems, root and root tuber in 1~2 h changed a little compared with 0 h under PEG- 6000 treatment. Under NaCl treatment, the expression of MeBADH1 in leaf at 8 h was the highest, and was extremely significantly higher than that at 0 h (P＜0.01); the expression of MeBADH2 did not change much at different times. In stems and roots, the expressions of MeBADH1 and MeBADH2 at 1 h were significantly higher than that at 0 h (P＜0.01). Under natural drought treatment, the expressions of MeBADH1 and MeBADH2 in stem and root at 6 and 18 d were significantly higher than that at 0 d (P＜0.01), respectively. Under PEG-6000 treatment, the GB content was fluctuant and slightly increased. The GB content under NaCl treatment showed stable expression in the early stage, and then occurred intense response at 8 h (appeared downward trend), and the GB content at 24 h was significantly lower than that at 0 h (P＜0.05). Under natrual drought stress, the GB content gradually increased at beginning, reached he peak at 9 and 15 d, and was higher than that st 0 d (P＜0.05). This study provides foundation for further research on the molecular mechanisms of cassava resistance to drought stress and breeding drought-resistant new cultivars.
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Received: 04 January 2017
Published: 20 July 2017
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