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| Effect of Inhibition of StOSM-3b Expression on Phenotypy in Potato(Solanum tuberosum) |
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Abstract Abstract Osmotin (OSM) is a multifunction protein present in wide plant species. It is inferred that OSM regulates plant adaption to stress through involving in plant growth and development, However, direct evidence linking between OSM and growth is rare. Based on StOSM-3b cDNA of potato (Solanum tuberosum) isolated in previous study, an antisense StOSM-3b vector, aiming to inhibit OSM expression, was constructed in this study. The antisense StOSM-3b was transformed into potato by Agrobacterium-mediated approach. A positive transgenic line was screened by β- glucuronidase (GUS) staining. Analysis on data assayed showed that accumulations of StOSM-3b mRNA and OSM contents by enzyme linked immunosorbent assay (ELISA) in the transgenic potato leafs was 25% and 52% of that in control, respectively. This indicated that the antisense StOSM-3b partially inhibited expression of StOSM-3b and down-regulated OSM accumulation in the transgenic potato. OSM down-expression led to a tardy growth, dwarfing and no tuber-formation phenotype with disorder in growth rhythm. The results provide a direct evidence to indicate that osmotin involves in plant growth and development.
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Received: 15 February 2017
Published: 20 July 2017
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