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| Cloning of GhJAZ10 Gene from Cotton and Its Response to Drought Stress |
| LI Dong-Liang1, WANG Cai-Xiang2*, ZHAO Ge2, XIAO Yu2, GE Xiao-Yang2, LI Fu-Guang2, YANG Qing-Hua1* |
1 College of Agriculture, Henan Agricultural University, Zhengzhou 450000, China;
2 State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China |
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Abstract JAZ (jasmonate ZIM-domain) is a major regulator of jasmonic acid signaling pathway and plays an important role in regulating plant development and responding to biotic and abiotic stresses. In the present study, GhJAZ10 (GenBank No. XM_016834302.1) was cloned from upland cotton (Gossypium hirsutum) 'zhongmiansuo 24' (CRI24), which was 723 bp in full length and encoded 240 amino acids which contained TIFY and Jas conserved domains. Subcellular localization by fusion of GFP reporter gene and transient transformation of tobacco (Nicotiana tabacum) showed that GhJAZ10 localized in the nucleus. qRT-PCR was used to analyze JAZ10 expression pattern in different tissues and different cultivars treated with PEG6000. The results showed that JAZ10 was highly expressed in flower tissues, roots and stems, and PEG6000 treatment up-regulated JAZ10 expression in tetraploid upland cotton (CRI24 and upland cotton genetic standard line TM-1), diploid cotton (Gossypium arboretum) 'Shixiya1' and wild-type diploid cotton (Gossypium raimondii). The gene was transformed into Arabidopsis thaliana by the floral dip method, and the homozygous transgenic line was obtained by resistance screening. The results showed that the stomata number of the transgenic line was significantly reduced compared with the wild type, root length and fresh weight increased significantly under drought conditions in transgenic lines, and stress response genes such as DREB2A (dehydration-responsive element binding protein 2A), RD22 (responsive to desiccation 22), DREB1A and DREB1B were up-regulated. This study preliminarily demonstrates the relationship between GhJAZ10 and drought stress response at the molecular level, and provides a theoretical reference for further exploration of its molecular mechanism in drought resistance.
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Received: 21 December 2018
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Corresponding Authors:
wangcaixiang@caas.cn; yangqh2000@163.com
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[1] Browse J.2005. Jasmonate: An oxylipin signal with many roles in plants[J]. Vitamins & Hormones-advances in Research & Applications, 72: 431-456.
[2] Chen X, Huang H, Qi T, et al.2016. New perspective of the bHLH-MYB complex in jasmonate-regulated plant fertility in Arabidopsis[J]. Plant Signaling & Behavior, 11(2): e1135280.
[3] Chini A.2010. The ZIM domain mediates homo- and heteromeric interactions between Arabidopsis JAZ proteins[J]. The Plant Journal, 59(1): 77-87.
[4] Chini A, Boter M, Solano R.2010. Plant oxylipins: COI1/JAZs/MYC2 as the core jasmonic acid-signalling module[J]. FEBS Journal, 276(17): 4682-4692.
[5] Chini A, Fonseca S, Fernández G, et al.2007. The JAZ family of repressors is the missing link in jasmonate signalling[J]. Nature, 448(7154): 666-671.
[6] Chung H S, Niu Y, Browse J, et al.2009. Top hits in contemporary JAZ: An update on jasmonate signaling[J]. Phytochemistry, 70(13-14): 1547-1559.
[7] Clough S J, Bent A F.2010. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana[J]. The Plant Journal, 16(6): 735-743.
[8] Fernández-Calvo, Solano P, Roberto.2011. The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of JAZ repressors and act additively with MYC2 in the activation of jasmonate responses[J]. Plant Cell, 23(2): 701-715.
[9] He X, Zhu L, Wassan G M, et al.2017. GhJAZ2 attenuates cotton resistance to biotic stresses via inhibiting the transcriptional activity of GhbHLH171[J]. Molecular Plant Pathology, 19(4): 896-908.
[10] Hu H, He X, Tu L, et al.2016. GhJAZ2 negatively regulates cotton fiber initiation by interacting with the R2R3-MYB transcription factor GhMYB25-like[J]. The Plant Journal, 88(6): 921-935.
[11] Hu Y, Jiang L, Wang F, et al.2013. Jasmonate regulates the inducer of cbf expression-C-repeat binding factor/DRE binding factor1 cascade and freezing tolerance in Arabidopsis[J]. Plant Cell, 25(8): 2907-2924.
[12] Huang H, Gao H, Liu B, et al.2017. Arabidopsis MYB24 regulates jasmonate-mediated stamen development[J]. Frontiers in Plant Science, 8: 1525-1532.
[13] Jiang Y, Liang G, Yang S, et al.2014. Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence[J]. Plant Cell, 26(1): 230-245.
[14] John B, Howe G A.2008. New weapons and a rapid response against insect attack[J]. Plant Physiology, 146(3): 832-838.
[15] Laurens P, Gemma Fernández B, Jan G, et al.2010. NINJA connects the co-repressor TOPLESS to jasmonate signalling[J]. Nature, 464(8): 788-791.
[16] Li W, Xia X C, Han L H, et al.2017. Genome-wide identification and characterization of JAZ gene family in upland cotton (Gossypium hirsutum)[J]. Scientific Reports, 7(1): 2788-2802.
[17] Livak K J, Schmittgen T D.2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 25(4): 402-408.
[18] Lv Y, Yang M, Hu D, et al.2017. The OsMYB30 transcription factor suppresses cold tolerance by interacting with a JAZ protein and suppressing β-amylase expression[J]. Plant Physiology, 173(2): 1475-1491.
[19] Michael R, Rohit D, Mohamed H, et al.2015. Exploring jasmonates in the hormonal network of drought and salinity responses[J]. Frontiers in Plant Science, 6: 1077-1093.
[20] Pauwels L, Barbero G F, Geerinck J, et al.2010. NINJA connects the co-repressor TOPLESS to jasmonate signalling[J]. Plant Signaling & Behavior, 464(8): 788-791.
[21] Qi T, Song S, Ren Q, et al.2011. The Jasmonate-ZIM-domain proteins interact with the WD-Repeat/bHLH/MYB complexes to regulate Jasmonate-mediated anthocyanin accumulation and trichome initiation in Arabidopsis thaliana[J]. Plant Cell, 23(5): 1795-1814.
[22] Qu L J, Zhao Y.2011. Plant hormones: Metabolism, signaling and crosstalk[J]. Journal of Integrative Plant Biology, 53(6): 410-411.
[23] Seo J S, Joo J, Kim M J, et al.2011. OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice[J]. The Plant Journal, 65(6): 907-921.
[24] Song S, Qi T, Huang H, et al.2011. The jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect jasmonate-regulated stamen development in Arabidopsis[J]. Plant Cell, 23(3): 1000-1013.
[25] Sun H, Chen L, Li J, et al.2017. The jasmonate ZIM-domain gene family mediates JA signaling and stress response in cotton[J]. Plant & Cell Physiology, 58(12): 2139-2154.
[26] Taniguchi S, Hosokawa-Shinonaga Y, Tamaoki D, et al.2014. Jasmonate induction of the monoterpene linalool confers resistance to rice bacterial blight and its biosynthesis is regulated by JAZ protein in rice[J]. Plant Cell & Environment, 37(2): 451-461.
[27] Thines B, Katsir L, Melotto M, et al.2007. JAZ repressor proteins are targets of the SCF (COI1) complex during jasmonate signalling[J]. Nature, 448(7154): 661-665.
[28] Thireault C, Shyu C, Yoshida Y, et al.2015. Repression of jasmonate signaling by a non-TIFY JAZ protein in Arabidopsis[J]. The Plant Journal, 82(4): 669-679.
[29] Wang Y, Qiao L, Bai J, et al.2017. Genome-wide characterization of JASMONATE-ZIM DOMAIN transcription repressors in wheat (Triticum aestivum L.)[J]. BMC Genomics, 18(1): 152-171.
[30] Wasternack C, Hause B.2013. Jasmonates: Biosynthesis, perception, signal transduction and action in plant stress response, growth and development. An update to the 2007 review in Annals of Botany[J]. Annals of Botany, 111(6): 1021-1058.
[31] Yamada S, Kano A, Tamaoki D, et al.2012. Involvement of OsJAZ8 in jasmonate-induced resistance to bacterial blight in rice[J]. Plant and Cell Physiology, 53(12): 2060-2072.
[32] Yamaguchi-Shinozaki K, Shinozaki K.1993. The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana[J]. Molecular Genetics & Genomics, 238(1-2): 17-25.
[33] Yan Y, Stolz S, Chételat A, et al.2007. A downstream mediator in the growth repression limb of the jasmonate pathway[J]. Plant Cell, 19(8): 2470-2483.
[34] Zhai Q, Zhang X, Wu F, et al.2015. Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis[J]. Plant Cell, 27(10): 2814-2828.
[35] Zhang X, Yao D, Wang Q, et al.2013. mRNA-seq analysis of the Gossypium arboreum transcriptome reveals tissue selective signaling in response to water stress during seedling stage[J]. PLoS One, 8(1): e54762.
[36] Zhao G, Song Y, Wang C, et al.2016. Genome-wide identification and functional analysis of the TIFY gene family in response to drought in cotton[J]. Molecular Genetics & Genomics, 291(6): 2173-2187. |
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