Cloning of GhJAZ10 Gene from Cotton and Its Response to Drought Stress
LI Dong-Liang1, WANG Cai-Xiang2*, ZHAO Ge2, XIAO Yu2, GE Xiao-Yang2, LI Fu-Guang2, YANG Qing-Hua1*
1 College of Agriculture, Henan Agricultural University, Zhengzhou 450000, China; 2 State Key Laboratory of Cotton Biology/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
Abstract JAZ (jasmonate ZIM-domain) is a major regulator of jasmonic acid signaling pathway and plays an important role in regulating plant development and responding to biotic and abiotic stresses. In the present study, GhJAZ10 (GenBank No. XM_016834302.1) was cloned from upland cotton (Gossypium hirsutum) 'zhongmiansuo 24' (CRI24), which was 723 bp in full length and encoded 240 amino acids which contained TIFY and Jas conserved domains. Subcellular localization by fusion of GFP reporter gene and transient transformation of tobacco (Nicotiana tabacum) showed that GhJAZ10 localized in the nucleus. qRT-PCR was used to analyze JAZ10 expression pattern in different tissues and different cultivars treated with PEG6000. The results showed that JAZ10 was highly expressed in flower tissues, roots and stems, and PEG6000 treatment up-regulated JAZ10 expression in tetraploid upland cotton (CRI24 and upland cotton genetic standard line TM-1), diploid cotton (Gossypium arboretum) 'Shixiya1' and wild-type diploid cotton (Gossypium raimondii). The gene was transformed into Arabidopsis thaliana by the floral dip method, and the homozygous transgenic line was obtained by resistance screening. The results showed that the stomata number of the transgenic line was significantly reduced compared with the wild type, root length and fresh weight increased significantly under drought conditions in transgenic lines, and stress response genes such as DREB2A (dehydration-responsive element binding protein 2A), RD22 (responsive to desiccation 22), DREB1A and DREB1B were up-regulated. This study preliminarily demonstrates the relationship between GhJAZ10 and drought stress response at the molecular level, and provides a theoretical reference for further exploration of its molecular mechanism in drought resistance.
LI Dong-Liang,WANG Cai-Xiang,ZHAO Ge, et al. Cloning of GhJAZ10 Gene from Cotton and Its Response to Drought Stress[J]. 农业生物技术学报, 2019, 27(7): 1179-1189.
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