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| Isolation and Identification of Serratia sp. Strain BRC-CXG2 and Synergism of Its Crude Extraction to Bacillus thuringiensis |
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Abstract Serratia sp., one of the opportunistic pathogen, possesses certain insecticidal activity against insect. In this study, Serratia sp. strain BRC-CXG2 (KT366770) was isolated from the corpse of Monochamus alternatus Hope which was suffered from sepsis and was identified through morphological identification, 16S rDNA phylogenetic analysis, physiological and biochemical reactions, drug sensitive test and biometric identification. The results showed that the strain BRC-CXG2 belonged to one of new species of Serratia sp. and possessed different levels of sensitivity to a number of common medicines, such as cefoperazone, cefotaxime and ceftazidime. In addition, the prodigiosin isolated from this strain increased significantly the toxicity of spore and crystal mixture produced by Bacillus thuringiensis israelensis (Bti) LLP29 to Aedes aegypti. The research provides a theoretical basis for the effective development of Bt synergists (cotoxicity coefficient: 128.06), and construction of new genetically engineered bacteria.
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Received: 08 June 2015
Published: 05 October 2015
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| [1] 吕志平, 潘德观.登革热的流行概况[J].中国国境卫生检疫杂志. 1999(04).[2]Arvind SHARMA, Richa SHARMA, Ashwani KUMAR et al.Gene flow and population genetic structure of Anopheles stephensi (Diptera: Culicidae) in the areas under Enhanced Malaria Control Project (EMCP) and non-EMCP of Madhya Pradesh,Central India using microsatellite markers(In English)[J].Acta Entomologica Sinica, 2015, 58(1):74-81[3]K.Raghvendra, S.K. Subbarao, Chemical Insecticides in malaria vector control in India, ICMR Bull. 32 (10) (2002) 94–99.[4]WEI Ji-bo, LI Gui-ling, MENG Qing-dong et al.Efficacy of Bacillus thuringiensis serotype H-14 in controlling vector mosquitoes[J].Chines Journal of Vector Biology and Control, 2014, 25(6):502-505[5]王美秀.微生物制剂杀灭蚊幼虫的应用前景[J]. 中国媒介生物学及控制杂志. 1995(05).[6] 刘娥英, 周志宏, 张用梅.球形芽孢杆菌C_（3－41）菌株杀蚊毒蛋白的生物合成[J].中国媒介生物学及控制杂志.1995(05).[7]张灵玲, 关怡, 张群林等.苏云金芽孢杆菌杀蚊机制研究概况[J].中国媒介生物学及控制杂志, 2008, 19(4):377-378[8]Entwistle P F, Corey J S, Bayley M Tet al.Bacillus thuringiensis, an environmental biopesticide:theory and practice. 1993.[9]ARONSON A I, SHAI Y.WhyBacillus thuringiensisinsecticidal toxins are so effective:unique features of their mode of action. FEMS Microbiology Letters . 2001.[10]Eisenstein BI (1990).Enterobacteriaceae. In: Mandell GL, Douglas RG, Bennett JE, Eds. Principles and practice of infectious disease 3rd ed. Churchill Livingstone New York, pp. 1658-1673.[11]杨玉英, 曹涤非, 王璐璐等.饮用纯净水中粘质沙雷氏菌的分离及其生物学特性研究[J].黑龙江八一农垦大学学报. 2006(05).[12]李晓虹, 黄逸男, 韩伟等.乳制品中粘质沙雷菌的分子生物学检测方法研究[J]. 中国卫生检验杂志. 2009(03).[13]程瑞春, 崔建国, 王洪魁等.柞蚕蛹期灵菌败血病 菌株分离鉴定[J].微生物学通报, 2010, 37(6):0829-0833[14]Jurat-Fuentes, J.L., Jackson, T.A., 2012. Bacterial Entomopathogens. In: Vega, F.E., Kaya, H.K. (Eds.), Insect Pathology, second ed. Academic Press, London, UK, pp.265–349.[15]C.D. Patil, S.V. Patil, B.K. Salunke et al. Bioe?cacy of Plumbago zeylanica(Plumbaginaceae) and Cestrum nocturnum (Solanaceae) plant extracts against Aedes aegypti (Diptera: Culicide) and nontarget ?sh Poecilia reticulate, Parasitol. Res. 108 (2011) 1253–1263.[16]余建申, 吴大富, 卢建明.100例沙雷氏菌感染及药敏分析[J]. 实用医技杂志. 2005(22).[17]陈亚梅, 黄俊荣.家蚕细菌性败血病的发生与防治[J]. 云南农业科技. 2008(S2).[18]系统发育树的构建方法[J].微生物学报. 2010(09).[19]黄萍, 孙平平, 马雅楠等.系统发育谱构建方法研究[J]. 生物信息学. 2009(01).[20]Petti CA (2007).Detection and identification of microorganisms by gene amplification and sequencing. Clin. Infect. Dis. 44: 1108-1114.[21]Clarridge III JE (2004).Impact of 16s rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev. 17: 840-862.[22]陈宁涛, 王能超, 施保昌.创建neighbor-joining进化树的快速算法(英文)[J]. Journal of Southeast University(English Edition). 2006(02).[23]东秀珠, 蔡妙英等编著.常见细菌系统鉴定手册[M].科学出版社, 2001.[24]布坎南(R.E.Buchanan)等编, 中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译.伯杰细菌鉴定手册[M].科学出版社, 1984. |
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