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| Transform Ability and Individual Characteristics of an Atrazine-degrading Consortium NC1 |
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Abstract In order to select atrazine-degrading consortium and strain resources, in the present research, by enrichment culture, a stable 4-member bacterial consortium NC1 was isolated from long-term use of atrazine surface corn-planted soil (Nancha area, Yichun City, Heilongjiang Province, China). The consortium NC1 was attenuated and plated on mineral salts medium agar plates, strains were repeatedly crossed vaccination on mineral salts medium agar plates for separation of pure culture, 4 bacterial isolates, named strain YJY1, YJY2, YJY3 and YJY5. Based on physiological and biochemical tests, and 16S rDNA gene sequence analysis, 4 bacterial strains were shown to belong to the Gram-negative species Enterobacter sp., Achromobacter xylosoxidans, Pseudomonas aeruginosa and Klebsiella pneumonia, respectively. These species were not stated previously as being capable of atrazine-degrading. By growth capacity measuring, consortium NC1, strain YJY5 could use atrazine as single nitrogen source for growth, and strain YJY1, YJY2 and YJY3 could not. Consortium NC1 and 4 strains could hardly grow in MSM with cyanuric acid as the sole nitrogen source. Consortium NC1 and strain YJY5 had a short lag period (8~10 h), then was logarithmic phase. Strain YJY5 was stagnate phase of 28~36 h, and consortium NC1 was still growing rapidly at 36 h and capable of degrading 100 mg/L atrazine to nontoxic cyanuric acid. Altogether this was a new combination of isolates in an atrazine- degrading consortium, and its degradation rate and the growth were both faster than that of the sole strain. The high atrazine degradation ability of the consortium NC1 showed good potential for atrazine biodegradation. This research will contribute toward a better understanding of the metabolic activities of atrazine-degrading consortium, which are generally considered to be responsible for atrazine mineralization in the natural environment. The results provide information and reference for the study on biodegradation of atrazine work.
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Received: 07 April 2015
Published: 05 October 2015
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