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Isolation and Identification of Serratia sp. Strain BRC-CXG2 and Synergism of Its Crude Extraction to Bacillus thuringiensis |
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Abstract Serratia sp., one of the opportunistic pathogen, possesses certain insecticidal activity against insect. In this study, Serratia sp. strain BRC-CXG2 (KT366770) was isolated from the corpse of Monochamus alternatus Hope which was suffered from sepsis and was identified through morphological identification, 16S rDNA phylogenetic analysis, physiological and biochemical reactions, drug sensitive test and biometric identification. The results showed that the strain BRC-CXG2 belonged to one of new species of Serratia sp. and possessed different levels of sensitivity to a number of common medicines, such as cefoperazone, cefotaxime and ceftazidime. In addition, the prodigiosin isolated from this strain increased significantly the toxicity of spore and crystal mixture produced by Bacillus thuringiensis israelensis (Bti) LLP29 to Aedes aegypti. The research provides a theoretical basis for the effective development of Bt synergists (cotoxicity coefficient: 128.06), and construction of new genetically engineered bacteria.
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Received: 08 June 2015
Published: 05 October 2015
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