Genetic Effect of Tyrosinase (TYR), Microphthalmia-associated Transcription Factor (MITF) and Agouti Signaling Protein (ASIP) Genes on Melanin Deposition of White Silky Fowl (Gallus gallus domesticus Brisson)
Abstract In order to study the molecular mechanism of melanin deposition in White Silky Fowl (Gallus gallus domesticus Brisson), the quantitative Real-time PCR (qRT-PCR) was used to detect the expression level of tyrosinase (TYR), agouti signaling protein (ASIP) and microphthalmia-associated transcription factor(MITF) genes in the skin, muscle, liver, kidney and gizzard of White Silky Fowl and the ultraviolet spectrophotometry was adopted to measure the melanin content in tissues for exploring the relationship between mRNA expression of the 3 genes and melanin deposition in tissues of White Silky Fowl. Results showed that the mRNA of TYR, MITF and ASIP expressed very significantly differently in all test tissues of White Silky Fowl (P＜0.01) with an order of skin＞kidney＞gizzard＞liver＞muscle of MITF and TYR, which were consistent with the law of melanin deposition in White Silky Fowl. However, the mRNA expression of ASIP in tissues showed a contrary trend of muscle＞liver＞gizzard＞kidney＞skin. Correlation analysis indicated that the mRNA expression of TYR and MITF were significently positively correlated with the melanin content in tissues of White Silky Fowl (P＜0.05), while that of ASIP showed significently negative correlation (P＜0.05). It was speculated that the expression of TYR, MITF and ASIP had certain correlation with the melanin deposition in White Silky Fowl, respectively. High expression of TYR and MITF might promote melanin deposition while ASIP might inhibit melanin deposition in White Silky Fowl. The results would provide a theoretical basis for furtherly elucidating the genetic regulation mechanism of melanin deposition.
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