|
|
Expression Analysis for Growth Hormone Receptor (GHR) in Different Tissues of Yak (Bos grunneins) |
|
|
Abstract To explore the expression of growth hormone receptor (GHR) in different tissues of yak(Bos grunneins), samples of these tissues (heart, liver, spleen, lung, kidney and testis) were collected from healthy yaks under normal physiological conditions. Quantitative Real-time PCR (qRT-PCR) was applied to analyze the expression of GHR gene in different tissues. Immunohistochemistry (IHC) was used to detect the distribution and localization of GHR protein, integrated optical density (IOD) of GHR immunoreactions were measured, and followed by an analysis for significant difference. The results of qRT-PCR showed that GHR gene had a differential expression in different tissues, comparative expression quantity of GHR gene in liver was 3.73 fold than that in heart, 4.98 fold in kidney, 5.03 fold in testis, 8.58 fold in lung and 10.21 fold in spleen, respectively. Unequal immunoreaction intensity was observed in different tissues by immunohistochemistry. Comparative analysis of IOD showed that GHR had the strongest immunoreactions in liver, and followed by the heart, kidney, testis and spleen, the weakest immunoreactions of GHR was found in lung. This experiment demonstrated that GHR had a differential expression in different tissues by studying at genetic and protein level. This work provides a theoretical information for improvement and breeding of yak.
|
Received: 29 December 2014
Published: 09 July 2015
|
|
|
|
[1]ARGETSINGER L S, CARTER-SU C.Mechanism of signaling by growth hormone receptor[J].Physiological Reviews, 1996, 76(4):1089-1107
[2]孙逊, 朱尚权.生长激素受体的结构, 功能及其信号途径[J].国外医学: 生理病理科学与临床分册, 1999, 19(1):9-14
[3]TSUSHIMA T, FRIESEN H G.Radioreceptor assay for growth hormone[J].The Journal of Clinical Endocrinology & Metabolism, 1973, 37(2):334-337
[4]WATERS M J, FRIESEN H G.Purification and partial characterization of a nonprimate growth hormone receptor[J].Journal of Biological Chemistry, 1979, 254(14):6815-6825
[5]焦凯, 史轶繁.生长激素受体[J].国外医学: 内分泌学分册, 1993, 13(2):58-59
[6]WALLIS M.Growth hormone receptor cloned[J].Nature, 1987, 330(6148):521-522
[7]ILKBAHAR Y N, WU K, THORDARSON G, et al.Expression and distribution of messenger ribonucleic acids for growth hormone (GH) receptor and GH-binding protein in mice during pregnancy[J].Endocrinology, 1995, 136(2):386-392
[8]MERTANI H C, DELEHAYE-ZERVAS M C, MARTINI J F, et al.Localization of growth hormone receptor messenger RNA in human tissues[J].Endocrine, 1995, 3(2):135-142
[9]TIONG T S, FREED K A, HERINGTON A C.Identification and tissue distribution of messenger RNA for the growth hormone receptor in the rabbit[J].Biochemical and biophysical research communications, 1989, 158(1):141-148
[10]SHEN X Y, HOLT R I G, MIELL J P, et al.Cirrhotic Liver Expresses Low Levels of the Full-Length and Truncated Growth Hormone Receptors 1[J].The Journal of Clinical Endocrinology & Metabolism, 1998, 83(7):2532-2538
[11]GARCíA-CABALLERO T, MERTANI H M, LAMBERT A, et al.Increased expression of growth hormone and prolactin receptors in hepatocellular carcinomas[J].Endocrine, 2000, 12(3):265-271
[12]CHANG T C, LIN J J, YU S C, et al.Absence of growth‐hormone receptor in hepatocellular carcinoma and cirrhotic liver[J].Hepatology, 1990, 11(1):123-126
[13]SCHWARTZBAUER G, CHENG H, MENON R K, et al.Expression of novel growth hormone receptor (GHR) transcripts in human heart tissue[J].Growth Hormone & IGF Research, 1998, 8(4):328-328
[14]LU C, SCHWARTZBAUER G, SPERLING M A, et al.Demonstration of direct effects of growth hormone on neonatal cardiomyocytes[J].Journal of Biological Chemistry, 2001, 276(25):22892-22900
[15]KATSUMATA M, CATTANEO D, WHITE P, et al.Growth hormone receptor gene expression in porcine skeletal and cardiac muscles is selectively regulated by postnatal undernutrition[J].The Journal of nutrition, 2000, 130(10):2482-2488
[16]HULL K L, THIAGARAJAH A, HARVEY S.Cellular localization of growth hormone receptors in immune tissues[J].Cell and tissue research, 1996, 286(1):69-80
[17]HULL K L, HARVEY S.Autoregulation of growth hormone receptor and growth hormone binding protein transcripts in brain and peripheral tissues of the rat[J].Growth Hormone & IGF Research, 1998, 8(2):167-173
[18]BEYEA J A, SAWICKI G, OLSON D M, et al.Growth hormone (GH) receptor knockout mice reveal actions of GH in lung development[J].Proteomics, 2006, 6(1):341-348
[19]BEYEA J A, OLSON D M, VANDERGRIEND R A, et al.Expression of growth hormone and its receptor in the lungs of embryonic chicks[J].Cell and tissue research, 2005, 322(3):379-392
[20]CHENG C Y, PEREVEDENTSEVA E, TU J S, et al.Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling[J].Applied physics letters, 2007, 90(16):163903-163903
[21]LANDAU D, DOMENE H, FLYVBJERG A, et al.Differential expression of renal growth hormone receptor and its binding protein in experimental diabetes mellitus[J].Growth Hormone & IGF Research, 1998, 8(1):39-45
[22]GIANI J F, MIQUET J G, MU?OZ M C, et al.Upregulation of the angiotensin-converting enzyme 2angiotensin-(1–7)Mas receptor axis in the heart and the kidney of growth hormone receptor knock-out mice[J].Growth Hormone & IGF Research, 2012, 22(6):224-233
[23]MARTíNEZ-MORENO C G, PALMA L, CARRANZA M, et al.Cellular and intracellular distribution of growth hormone in the adult chicken testis[J].General and comparative endocrinology, 2011, 172(3):344-357
[24]RABASSA V R, SCHWEGLER E, SCHMITT E, et al.Effect of porcine somatotropin on metabolism and testicular characteristics of prepubertal pigs[J].Brazilian Journal of Veterinary Research and Animal Science, 2014, 51(1):60-67
[25]K?LLE S, SINOWATZ F, BOIE G, et al.Developmental changes in the expression of the growth hormone receptor messenger ribonucleic acid and protein in the bovine ovary[J].Biology of reproduction, 1998, 59(4):836-842
[26]HRABIA A, PACZOSKA-ELIASIEWICZ H E, BERGHMAN L R, et al.Expression and localization of growth hormone and its receptors in the chicken ovary during sexual maturation[J].Cell and tissue research, 2008, 332(2):317-328
[27]ZHAO J, TAVERNE M A M, VAN DER WEIJDEN G C, et al.Immunohistochemical localisation of growth hormone (GH),GH receptor (GHR),insulin-like growth factor I (IGF-I) and type I IGF-I receptor,and gene expression of GH and GHR in rat pre-antral follicles[J].Zygote, 2002, 10(01):85-94
|
[1] |
ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
|
|
|
|