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| Polymorphism and Tissue Expression Pattern of Parathyroid Hormone-related Peptide Gene (PTHrP) in Gaojiao Chicken (Gallus) |
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Abstract In order to reveal the genetic diversity and tissue expression pattern of Parathyroid hormone-related peptide gene (PTHrP) in Gaojiao chickens (Gallus), Gaojiao chickens in Guizhou province were used in the present study, Aijiao chickens and Baiyi black chickens as controls. Its polymorphic loci were detected with DNA pool, Single Strand Conformation Polymorphism (SSCP) and DNA sequencing techniques, and its differential expression levels in heart, liver, breast muscl and leg muscle were analyzed by using real-time quantitative PCR technology. The results showed that the C→T point mutation was detected in 209bp of exon 3 of PTHrP gene of Gaojiao chickens，which was a silent mutation, hree genotypes (CC, CT and TT) were found.The relationship between PTHrP gene and shank length in Gaojiao chickens was analyzed. The C209T polymorphism wad significantly associate with shank length of Gaojiao chicken, and the individuals with CT genotype had significant shank length than CC and TT (P＜0.05). Thefitness test indicated that Gaojiao chickens at the mutation locus were Hardy-weinberg equilibrium(P＞0.05), and itpresented moderate polymorphism (PIC=0.350).. The RQ-PCR detection showed that the expression level of PTHrP gene was the highest in leg muscle of Gaojiao chickens, and significant higher than other tissues (P＜0.05). In addition, compare with Aijiao chicken and Baiyi black chicken,the expression levels of various tissues of Gaojiao chickens were higher than that of Baiyi black chickens (P＜0.05)，but lower than Aijiao chickens (P＜0.05). The results indicate that polymorphisms of PTHrP gene are significantly correlated with shank length traits of Gaojiao chickens, and individuals with CT genotype can be used as molecular genetic marker to select shank length of Gaojiao chicken for reproduction traits, and the expression levels of PTHrP has an influence on chickens' shank length.
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Received: 23 January 2015
Published: 01 June 2015
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