利用甲基磺酸乙酯(EMS)诱导小麦-华山新麦草染色体易位的研究

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Abstract The germplasm 24-3-1 is a wheat (Triticum aestivum)-Psathyrostachys huashanica disomic alien addition line which is selected by means of chromosome engineering, and has high resistance to wheat stripe rust. In order to improve its genetic stability, and promote the effective utilization of the agronomic genes of Psathyrostachys huashanica, 24-3-1 was treated by ethylmethylsulfone (EMS) for inducing translocation lines. M2 plants with translocation chromosomes were identified by cytology analysis and genomic in situ hybridization (GISH), and the inductive effect of different concentrations of EMS was analyzed. This study identified 61 plants with wheat-Psathyrostachys huashanica translocation chromosomes from 930 M2 plants, and the translocation frequency was 6.56%. Of the 61 plants, 7 plants were detected with 1 translocation chromosome and 5 plants with 1 translocation chromosome and 1 Psathyrostachys huashanica chromosome; in addition, 20 plants, 3 plants, and 26 plants were detected with 2, 3 and 4 translocation chromosomes, respectively. The cytological observation of root-tip cell and GISH analysis indicated that the plants with 2 translocation chromosomes were wheat-Psathyrostachys huashanica translocation lines; and the plants with 4 translocation chromosomes were wheat-Psathyrostachys huashanica translocation-translocation addition lines. This study also showed that 1.0% EMS was an optimal concentration to induce wheat-Psathyrostachys huashanica chromosome translocation. Furthermore, the research not only provides important genetic resources for wheat special genetic materials establishment, but also provides new germplasms in wheat breeding.

Key words： Wheat Ethylmethylsulfone (EMS) Wheat-Psathyrostachys huashanica Translocation lines Genomic in situ hybridization (GISH)

Received: 03 December 2014 Published: 17 March 2015

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	Articles by authors
	JING Fan
	YU Liang-Meng
	WU Jun
	DU Mo-Li
	YU Xiu-Juan
	SU Jia-Ni
	ZAN Kai
	DIAO Ji-Xin
	CHEN Xin-Hong

Cite this article:

JING Fan,YU Liang-Meng,WU Jun, et al. Study on Inducing Chromosome Translocation of Wheat (Triticum aestivum)-Psathyrostachys huashanica Using Ethylmethylsulfone (EMS)[J]. , 2015, 23(5): 561-570.

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http://journal05.magtech.org.cn/Jwk_ny/EN/ OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I5/561

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