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  2015, Vol. 23 Issue (5): 561-570    DOI:
Articles and Letters Current Issue | Archive | Adv Search |
Study on Inducing Chromosome Translocation of Wheat (Triticum aestivum)-Psathyrostachys huashanica Using Ethylmethylsulfone (EMS)
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Abstract  The germplasm 24-3-1 is a wheat (Triticum aestivum)-Psathyrostachys huashanica disomic alien addition line which is selected by means of chromosome engineering, and has high resistance to wheat stripe rust. In order to improve its genetic stability, and promote the effective utilization of the agronomic genes of Psathyrostachys huashanica, 24-3-1 was treated by ethylmethylsulfone (EMS) for inducing translocation lines. M2 plants with translocation chromosomes were identified by cytology analysis and genomic in situ hybridization (GISH), and the inductive effect of different concentrations of EMS was analyzed. This study identified 61 plants with wheat-Psathyrostachys huashanica translocation chromosomes from 930 M2 plants, and the translocation frequency was 6.56%. Of the 61 plants, 7 plants were detected with 1 translocation chromosome and 5 plants with 1 translocation chromosome and 1 Psathyrostachys huashanica chromosome; in addition, 20 plants, 3 plants, and 26 plants were detected with 2, 3 and 4 translocation chromosomes, respectively. The cytological observation of root-tip cell and GISH analysis indicated that the plants with 2 translocation chromosomes were wheat-Psathyrostachys huashanica translocation lines; and the plants with 4 translocation chromosomes were wheat-Psathyrostachys huashanica translocation-translocation addition lines. This study also showed that 1.0% EMS was an optimal concentration to induce wheat-Psathyrostachys huashanica chromosome translocation. Furthermore, the research not only provides important genetic resources for wheat special genetic materials establishment, but also provides new germplasms in wheat breeding.
Key wordsWheat      Ethylmethylsulfone (EMS)      Wheat-Psathyrostachys huashanica      Translocation lines      Genomic in situ hybridization (GISH)     
Received: 03 December 2014      Published: 17 March 2015
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Articles by authors
JING Fan
YU Liang-Meng
WU Jun
DU Mo-Li
YU Xiu-Juan
SU Jia-Ni
ZAN Kai
DIAO Ji-Xin
CHEN Xin-Hong
Cite this article:   
JING Fan,YU Liang-Meng,WU Jun, et al. Study on Inducing Chromosome Translocation of Wheat (Triticum aestivum)-Psathyrostachys huashanica Using Ethylmethylsulfone (EMS)[J]. , 2015, 23(5): 561-570.
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http://journal05.magtech.org.cn/Jwk_ny/EN/     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2015/V23/I5/561
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