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Abstract Start codon targeted polymorphism (SCoT) is a novel genetic marker technique. In this study, a single factor experiment method was designed to optimize and establish a SCoT molecular marker system for pear (Pyrus spp.). Furthermore, the diversity and genetic relationship of 43 pear cultivars, which were collected from Dangshan, Anhui Province, were analyzed with the optimized SCoT system. The results showed that the optimized SCoT-PCR in pear was as follows: A total volume of 25 μL-reaction system including 2.5 μL 10×Easy Taq buffer (with 2 mmol/L Mg2+), 30 mg/L template DNA, 1.0 μmol/L primer, 0.2 mmol/L dNTPs and 1.0 U Taq polymerase. Sixteen primer combinations were selected from 67 SCoT primers for their amplification with clear bands and significant difference. A total of 145 bands were amplified, in which 126 bands were polymorphic (86.1%) with 9.1 bands per primer on average. The optimized SCoT-PCR system was applied to 43 pear samples. The result showed abundant polymorphism in the tested pear. The value of genetic similarity coefficient was ranged from 0.517 to 0.931, with the average of 0.685. Moreover, the 43 pear varieties could be classified into 2 groups including A and B based on the similarity of 0.610 using the cluster analysis. When the genetic similarity coefficient was set at 0.746, group A could be divided into 6 subgroups (Ⅰ~Ⅵ). These results indicated that the tested 43 pear varieties had relatively high genetic diversity and could be distinguished from each other by SCoT molecular marker technology, which provide basis for further research and application on genetic resources in pear from Dangshan, Anhui province.
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Received: 13 November 2014
Published: 17 March 2015
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