Cloning and Expression Analysis of Potassium Channel Gene PbAKT1 in Pear (Pyrus betulifolia)
YANG Han1, LI Yan1, SHEN Chang-Wei2, JIN Yu-Meng1, SHI Xiao-Qian1, XIE Chang-Yan1, Mei Xin-Lan1, XU Yang-Chun1, DONG Cai-Xia1,*
1 Jiangsu Provincial Key Lab for Organic Solid Waste Utilization/ National Engineering Research Center for Organic-based Fertilizers/ Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization/ College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing,210095,China; 2 School of Resources and Environmental Sciences, Henan Institute of Science and Technology, Xinxiang 453003, China
AbstractAKT1 (Arabidopsis potassium transport 1) is an important potassium channel protein gene involved in plant potassium absorption and transport. In order to study the response and expression pattern of PbAKT1 gene in Pyrus betulifolia to potassium, this study adopted K+ at different concentrations (0.1 mmol/L and 3 mmol/L K+) to treat pear seedlings, cloned PbAKT1 gene and analyzed the tissue specificity of its encoded protein. Results showed that the PbAKT1 gene (GenBank No. MN150549) cDNA was 2 646 bp in length, encoding 881 amino acids. The gene had a transmembrane structure with a typical S1~S6 of the Shaker family and a loop ring. The homology analysis of amino acid sequence indicated that there was a close phylogenetic relationship between PbAKT1 gene and MdAKT1 gene (Malus domestica). Subcellular localization showed that the gene mainly presented in the cell membrane and was a hydrophilic stable protein. PbAKT1 gene was found no tissue specificity in roots, stems and leaves of pear seedlings and its expression obviously responded to low potassium stress. It was indicated that the expression of PbAKT1 was induced by potassium supply and might be involved in K+ absorption and transport of pear trees. It provides basic information for further research of the molecular mechanism to low potassium stress in pear.
YANG Han,LI Yan,SHEN Chang-Wei, et al. Cloning and Expression Analysis of Potassium Channel Gene PbAKT1 in Pear (Pyrus betulifolia)[J]. 农业生物技术学报, 2019, 27(8): 1341-1350.
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