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| Screening of Highly Efficient Cellulose Degradation Microbes and Construction of Composite Strains |
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Abstract Arming at screening mesophilic and high temperature resistant microbes of highly efficient cellulose-degradation and constructing composite strains for application as compost inoculant, the microbes were isolated using cellulose Congo red selective medium under room and high temperatures from humus soil and compost samples. Detections of carboxymethyl cellulase(CMCase) activity, filter paper degradation and degradation ability of rice straw and radix(Sophorae flavescens Ait.) residues under liquid and solid state fermentation conditions of isolated microbes were performed, and highly efficient microbes which were used to construct the composite strains were selected. The finally constructed lignocellulose degradation composite strains of high efficiency, which was determined by antagonism and single strain contribution among the composite strains analysis, contained 3 bacteria(Beijerinckia fluminensis, Microbacterium sp. and Bacillus sp.), 1 Streptomyces sp. and 1 Chaetomium sp.. Results showed that the degradation effects on rice straw and radix residues were stronger by the tested microbes under liquid state fermentation than in the solid state fermentation. The rice straw was more likely to be degraded by most of the tested strains than the radix residues, and the fungi species showed greater effects among the tested microbes in the rice straw and radix residues degradation. The Beijerinckia fluminensis strain XM-3 degraded the filter paper stripe completely within 48 h. The composite strains degraded 31.4% and 63.1% of the radix residues and rice straw after 20 d of solid state fermentation. This study provides reference for the development of compost inoculants.
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Received: 02 December 2014
Published: 01 March 2015
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