Abstract Piriformospora indica, isolated from Indian Thar desert, s identified as a plant root endophytic fungus which plays an important role in inducing resistance against biotic and abiotic stresses. Recently, P. indica has been regarded as a fungal model to study the interaction between endophytic fungus and plants. In order to study the influence of P.indica on black shank resistance in Nicotiana tobacum, we analyzed the differences of the incidence, antioxidant enzyme activities, including phenylalnine ammonialyase (PAL), peroxidase (POD) and polyphenol oxidasei(PPO), and the expression levels of disease resistance-related genes in P.indica-colonized and non-colonized plants after Phytophthora parasitica var. nicotianae inoculation. We also studied whether P.indica influenced the growth of P. parasitica var. nicotianae by antibiosis assay. The results showed that: (1)The disease severity of P. indica-colonized N. tobacum was significantly lower than that of non-colonized ones; (2) After P. parasitica var. nicotianae inoculation from 1 to 10 days, the activities of PAL, POD and PPO in P. indica-colonized tobacco were significantly higher than those in non-colonized plants. P. indica-colonized plants showed 3.99-fold greater PAL activity in comparison with controls after P.parasitica var. nicotianae inoculation for 1 day. Meanwhile, compared with controls, POD and PPO activity in P. indica-colonized plants were elevated by 1.5- and 2.26-fold after infestation with P. parasitica var. nicotianae for 2 and 4 days, respectively; (3) Real-time quantitative PCR (qRT-PCR) results showed that the expression levels of disease resistance-related genes (PR1b, P450-2, hrs203J, GST, NmIMSP and P450-1) were up-regulated in P. indica-colonized N. tobacum after P. parasitica var. nicotianae inoculation at 6 to 96 h, respectively. The expression levels of PR1b, P450-2, hrs203J were up-regulated by 3.8, 15.7 and 3.1 folds in P. indica-colonized N. tobacum compared with controls after P. parasitica var. nicotianae inoculation for 12 h, respectively. The NmIMSP expression reached a level of 2.6-fold higher in P. indica-colonized plants in contrast to the controls after P. parasitica var. nicotianae inoculation for 48 h. The P450-1 was 1.5 folds of the control ones after P. parasitica var. nicotianae inoculation for 72 h. (4) The antibiosis assay showed that P. indica didn’t inhibit P. parasitica var. nicotianae growth. Thus, the resistance induced by P.indica against P.parasitica var. nicotianae in N.tobacum was not dependent on the antibiotic secretion, but on the up-regulation of resistance-related genes and improved antioxidant enzyme activity in N. tobacum. This study reveals the role of P. indica conferring N. tobacum disease resistance against P. parasitica var. nicotianae and correlative mechanism, and provides a new way for the biological control of black shank.
PENG Bing,LIU Jian,HUI Fei-Qiong, et al. Piriformospora indica Inducing Tobacco Resistance to Black Shank and Its Preliminary Mechanism[J]. , 2015, 23(4): 432-440.
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