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Genetic Linkage Map Construction of Some Microsatellites on Pig
Chromosome 4 and 7 |
| Zuo Bo Xiong Yuanzhu Su Yuhong Deng Changyan Yu Li Zheng Rong |
| (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture, Huazhong Agriculture University, Wuhan 430070, China) |
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Abstract Abstract: A reference family was established by using three Large White boars (Sus scrofa )and seven Meishan dams(S.scrofa ). Blood samples of 140 F2 individuals were collected. Each sample was amplified by using primers of 7 microsatellites loci on chromosome 4 and 8 microsatellites loci on chromosome 7, and genotypes were identified. The linkage map of chromsome 4 and 7 was constructed by using CRIMAP software. In this population, average allele number was 3.6, average heterozygosity and polymorphic information content 0.531 and 0.463, average information meiosis 235. The genetic length of sex-average, female and male maps were 178.5, 197.7 and 172.2 cM for chromosome 4, and 179.1, 195.7 and 167.7 cM for chromosome 7 respectively. The average marker interval of chromosome 4 and 7 was 29.9 and 24.9 cM, respectively. Construction of linkage map will make the foundation for QTL mapping afterwards.
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Received: 01 January 1900
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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