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Construction and Expression of the Specific Secretory Vector
in the Chicken Oviduct Cells |
| Sun Mingjun Zhao Chen Sha Jin Han Haitang Zheng Kejian Li Zandong** |
| ( College of Biology, China Agricultural University, Beijing 100094，China) |
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Abstract Abstract:To express foreign proteins in chicken (Gallus gallus ) oviduct cells and secret to oviduct, 450 bp chicken lysozyme cDNA fragment was cloned by using RT-PCR method. Treated by the restriction endonuclease Bam HⅠand Hind Ⅲ, the fragment was ligated with the vector generated by the same Bam HⅠ-HindⅢ digestion of plasmid pOVA-GFP. The resulting expressive vector pOVALG included an 1.2 kb chicken ovalbumin 5'-flanking regulatory region and a lysozyme-GFP fusion gene with the signal peptide of the lysozyme. After transfecting the vector to the chicken embryo fibroblasts and mammary cancer cell line of rat SHZ-88 cells, green fluorescent protein had been detected successfully.
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Received: 01 January 1900
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