PDK1、HIF-1α 及 VEGF 在不同年龄牦牛肺脏中的表达差异

doi:10.3969/j.issn.1674-7968.2023.08.006

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Abstract Pyruvate dehydrogenase kinase 1 (PDK1) could be induced by hypoxia inducible factor 1α (HIF-1α) and participated in the regulation of glycolysis. The expression of vascular endothelial growth factor (VEGF) which is the target gene of HIF-1α up-regulated by PDK1. Furthermore, the PDK1 could promote the remodeling of lung structure. To reveal the expression and distribution characteristics of PDK1, HIF-1α and VEGF in the lungs of yaks (Bos grunniens) at different ages, and to explore their possible regulatory role in the adaptation of yak lungs to the hypoxia environment of the plateau, in this study, the lung tissues of healthy yaks aged newborn, 6-month-old, 3-year-old, and 6-year-old were collected. Hematoxylin-eosin (HE) staining, immunohistochemical staining, optical density analysis, qRT-PCR and Western blot were used to investigate the distribution and expression of PDK1, HIF-1α and VEGF in lung tissues of yaks in different age groups. The results of HE staining showed that the smooth muscle of the terminal bronchiole wall was thickened with age. The immunohistochemical and densitometric analysis results showed that PDK1, HIF-1α and VEGF were mainly distributed in the single-layer ciliated columnar epithelium of terminal bronchioles and their branches, as well as in the associated arteries and alveolar walls of yaks in different ages. Moreover, qRT-PCR results showed that the relative expressions levels of PDK1 and HIF-1α were the highest in the 6-year-old group, followed by the 3-year-old group. And there was no significant difference in the relative expression of VEGF among the 4 age groups. Additionally, the Western blot results showed that the expression of PDK1 protein in the 6-year-old group was significantly lower than that in newborn, 6-month-old and 3-year-old groups. The expression of HIF-1α and VEGF protein in the 6-year-old group was significantly higher than that in the newborn, 6-month-old and 3-year-old groups. Consequently, the protein expression of PDK1, HIF-1α and VEGF were negatively correlated with age. Expression of PDK1 increased and then decreased with age, while HIF-1α and VEGF showed an increasing trend. It was speculated that PDK1, HIF-1α and VEGF proteins could regulate glucose metabolism and vascular remodeling processes in the lung under hypoxia. In conclusion, PDK1, HIF-1α and VEGF might play an important role in the development of yaks lung and participate in the regulation of yaks adaptation to plateau hypoxia environment. This study provides basic data for comparative study of the adaptation mechanism in yaks of different ages to high altitude hypoxia.

Key words： Yak Lung Pyruvate dehydrogenase kinase 1 (PDK1) Hypoxia inducible factor 1α (HIF-1α) Vascular endothelial growth factor (VEGF)

Received: 11 May 2022

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S823.8+5

Corresponding Authors: * 186152592@xbmu.edu.cn

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	ZHANG Yi-Yang
	CHEN Shu-Wu
	LI Si-Jia
	LI Rui
	ZHOU Man-Lin
	YANG Kun
	ZHANG Lan
	YAO Yi-Fan
	ZHANG Qian
	QIAO Zi-Lin

Cite this article:

ZHANG Yi-Yang,CHEN Shu-Wu,LI Si-Jia, et al. Differential Expression of PDK1, HIF-1α and VEGF in the Lung of Yak (Bos grunniens) at Different Age[J]. 农业生物技术学报, 2023, 31(8): 1623-1631.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.08.006 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I8/1623

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