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| Expression Pattern and Subcellular Localization of the OFP Gene Family in Chinese Fir (Cunninghamia lanceolata) |
| YING Wei-Yang, HU Xian-Ge, HUANG Hua-Hong*, LIN Er-Pei, TONG Zai-Kang |
| The State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Lin'an 311300, China |
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Abstract The OVATE family protein (OFP) is a plant-specific regulatory protein that plays a significant role in regulating plant morphogenesis and secondary cell wall formation. However, little information about the OFP gene family in Chinese fir (Cunninghamia lanceolata) is available. In this study, ClOFP genes were identi-fied based on the full-length transcriptome. Bioinformatics characteristics and evolutionary relationships were analyzed using online service. Besides, relative expression patterns of all members in different tissues and or-gans were determined by real time fluorescence quantitative PCR (qRT-PCR). Furthermore, the subcellular lo-calizations of all proteins were determined by transient transfection assays. The results showed that 6 ClOFP genes were identified from RNA-seq data, with a typical OVATE domain and contained no introns. The phylo-![]() ![]() genetic analysis revealed that ClOFP were distributed in 5 subgroups. In addition, relative expression patterns of different tissues and organs varied differently. ClOFP1 was strongly expressed in young leaves and the rela-tive expressions in stems were approximately decreased with an increase of lignification. ClOFP2 dominantly expressed in female cone. ClOFP3 strongly expressed in root. The relative expression of ClOFP2/3 in low lig-nified stem (S1) was higher than that in others. ClOFP4 dominantly expressed in female cone, male cone and leaves. ClOFP5 was strongly expressed in male cone and the relative expressions of ClOFP5 in stems were ap-proximately increased with an increase of lignification. ClOFP6 was strongly expressed in stems and female cone. Moreover, the relative expressions of ClOFPs in leaves were decreased with an increase in maturity. The results of subcellular localization showed the localization of ClOFP1 in the cytoplasm, ClOFP2/3 in the plas-ma membrane and nuclear localization of ClOFP4/5/6. This suggested that ClOFPs might involve in different regulatory pathways in Chinese fir. Hence, ClOFPs might involve in regulating the development of cone, leaf, stem and other organs, as well as lignin synthesis. The research results provides a crucial theoretical basis for further research on organogenesis and wood formation of Chinese fir, At the same time, it also provides poten-tial genetic resources for molecular breeding of C. lanceolata.
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Received: 29 April 2022
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Corresponding Authors:
* huanghh@zafu.edu.cn
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