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| Application of Single-cell RNA Sequencing in the Study of Plant Tissues Development |
| MIAO Long1, WANG Wen-Hui1, HE Gen-Hua1, LI Jia-Jia1, GAO Hui-Hui1, WANG Xiao-Bo1*, QIU Li-Juan2* |
1 College of Agriculture, Anhui Agricultural University, Hefei 230036, China;
2 Institute of Crop Science, Chinese Academy of Agricultural Sciences / The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) /Key Laboratory of Crop Gene Resource and Germplasm Enhancement (MOA), Beijing 100081, China |
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Abstract Single-cell RNA sequencing (scRNA-seq) is an advanced technique for characterizing the expression profiles of heterogeneous cells in the same tissue, and plays a crucial role in the study of cell development trajectory. Compared with animal cells, the application of scRNA-seq in plants is still in its infancy, and has been gradually applied in Arabidopsis, rice (Oryza sativa) and maize (Zea mays). This review summarized the common techniques of scRNA-seq and their applications in the research of tissue development mechanism, cells dynamic development trajectory, direction of cells differentiation and interaction. Additionally, this article analyzed the problems, challenges and development prospects of the application of scRNA-seq in plants, hoping to provide a theoretical basis for explore the mechanism of plant tissue development.
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Received: 11 August 2022
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Corresponding Authors:
*wxbphd@163.com; qiulijuan@caas.cn
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