Identification and Expression Analysis of Homeodomain Family Genes in Botrytis cinerea
LI Bai1,2*, WEI Ya-Di2*, LIU Ying-Zi3, CAO Hong-Zhe2, ZHANG Kang1,2, XING Ji-Hong1,2**, DONG Jin-Gao1,2**
1 North China State Key Laboratry of Crop Improvement and Regulation, Jointly Established by the Province and the Ministry, Baoding 071000, China; 2 Hebei Provincial Key Laboratory of Plant Physiology and Molecular Pathology, Baoding 071000, China; 3 Landscape Management Center of Chengde City, Hebei Province, Chengde 067000, China
Abstract Homeodomain transcription factors are widely present in plant pathogenic fungi and play an important role in the morphogenesis and infestation of pathogens. In this study, bioinformatics was used to identify the Homeodomain family genes of Botrytis cinerea at the genome-wide level. The physicochemical properties, phylogenetic relationships, conserved structural domains and expression patterns during the developmental and infestation periods of the pathogenic conidia of Homeodomain were carried out. The relative expression of Homeodomain treated with NaCl and Congo red were analysed by qPCR. The results showed that there were 14 Homeodomain family genes in the genome of Botrytis cinerea, which were classified into 4 subfamilies by phylogenetic analysis; All the Homeodomain family genes of Botrytis cinerea contained the typical SANT domain of the Homeodomain family, and some of them were up-regulated during different developmental and infestation periods of the conidia. The expression levels of the Homeodomain family genes were significantly different after NaCl and Congo red treatment, indicating that the Homeodomain family genes played an important role in the growth, development, the process of infestation, and the response to stress of the pathogen. The present study provides a theoretical basis for the elucidation of the functions of the Homeodomain family genes and their mechanisms of action.
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