School of Landscape and Architecture/Zhejiang Key Laboratory of Garden Plant Germplasm Innovation and Utilization/ Key Laboratory of Southern Garden Plant Germplasm Innovation and Utilization, State Forestry and Grassland Administration, Zhejiang A&F University, Hangzhou 311300, China
Abstract Aquaporin (AQP) is a small molecule membrane intrinsic protein, which mediates the rapid and passive water transport and participates in transmembrane transport of plants. In this study, 41 genes of AQP gene family were identified according to non-redundant protein sequence database (Nr) annotation from the whole genome of Osmanthus fragrans. According to the analysis of amino acid sequence and phylogenetic tree, they were classified into 4 subgroups: 16 members of plasma membrane intrinsic proteins (PIP), 14 members of tonoplast intrinsic proteins (TIP), 8 members of nodulin-26-like intrinsic proteins (NIP), 3 members of small intrinsic proteins (SIP). The gene structure and conserved motif of amino acids in the same subgroup were highly conserved. The length of the AQP proteins ranged from 145 to 432 aa with molecular mass of 14.81 to 45.90 kD and the isoelectric points were from 4.84 to 9.81. The analysis of the expression patterns of OfPIPs and OfTIPs in different tissues of O. fragrans using qPCR showed that OfPIP1, OfPIP5, OfPIP12, OfPIP13, OfPIP14, OfPIP15, OfPIP16, OfTIP1, OfTIP3, OfTIP4, OfTIP6, OfTIP9 and OfTIP13 were specific expressed in flowers. Further study on their expression patterns during flower opening period of O. fragrans showed that, OfTIP6 was highly transcribed in the early flower opening period (S1~S2) of O. fragrans. It is speculated that OfTIP6 plays an important role in vacuole expansion at the early flower opening stage. However, the relative expression levels of OfPIP1, OfPIP13, OfPIP14, OfPIP15, OfPIP16 and OfTIP13 were significantly up-regulated at the late flower opening period (S4~S5) of O. fragrans (P<0.05). It is speculated that they are the key genes to exercise function at the late flower opening stage. This research provides a reference for further analyzing the biological function of OfAQPs protein.
ZHOU Jie,ZHONG Shi-Wei,FANG Qiu, et al. Identification of AQP Gene Family in Osmanthus fragrans and Its Expression Analysis During Flower Opening Stage[J]. 农业生物技术学报, 2023, 31(5): 943-957.
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