Transcriptome Analysis of Resistant and Susceptible Inbred Line of Maize (Zea mays) in Response to Early Infection of Cochliobolus heterostrophus
GAN Lin1, LAN Cheng-Zhong1, RUAN Miao-Hong2, LIU Xiao-Fei1, HUANG Wei-Qun2, DAI Yu-Li1,*, YANG Xiu-Juan1,*
1 Institute of Plant Protection/Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China; 2 Fujian Seed Station, Fuzhou 350001, China
Abstract Southern leaf blight was an important disease in maize planting region of the world. There were significant differences in resistance of maize (Zea mays) cultivar with different genetic backgrounds to the disease. In order to understand response profiles for gene expression in maize to early infection of Cochliobolus heterostrophus, transcriptional data in the resistant cultivar ('Mo17-R') and susceptible cultivar ('Luo31-S') of maize inbred line at 12 and 24 h after inoculation were analyzed using the transcriptome analyses. The results demonstrated that 11 416 and 11 027 differentially expressed genes (DEGs) were found in 'Mo17-R' and 'Luo31-S' after inoculation by C. heterostrophus, respectively. GO enrichment and KEGG metabolic pathway analysis revealed that the DEGs were most rich in the terms of cell, cell part, organelle, membrane, binding, catalytic activity, metabolic process, cellular process and single-organism process, which were also most rich in the pathways of phenylpropanoid biosynthesis, signal transduction, plant-pathogen interaction, starch and sucrose metabolism. Among the enriched pathways associated with resistance, the pathways of phenylpropanoid biosynthesis, plant hormone signal transduction and plant-pathogen interaction were common in 'Mo17-R' and 'Luo31-S', while the DEGs involved in terpenoid biosynthesis pathway were only enriched in 'Luo31-S'. Furthermore, theses DEGs related to peroxidase, cinnamyl alcohol dehydrogenase, ethylene-responsive transcription factor, cyclic nucleotide-gated ion channel, cytochrome P450 were specifically induced in the resistant inbred line ('Mo17-R'), while these DEGs were down-regulated or less induced in the susceptible inbred line ('Luo31-S'). It was speculated that these genes could play an important role in disease resistance for resistant maize at the early stage of C. heterostrophus infection. These results provides a theoretical basis for further exploitation of molecular mechanisms underlying the interaction between maize and C. heterostrophus, and maize resistance to the pathogen.
GAN Lin,LAN Cheng-Zhong,RUAN Miao-Hong, et al. Transcriptome Analysis of Resistant and Susceptible Inbred Line of Maize (Zea mays) in Response to Early Infection of Cochliobolus heterostrophus[J]. 农业生物技术学报, 2023, 31(3): 460-474.
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