Contact Us Add to Favorite
 
NianQi Search Adv Search
33
农业生物技术学报  2023, Vol. 31 Issue (4): 682-694    DOI: 10.3969/j.issn.1674-7968.2023.04.002
Articles and Letters Current Issue | Archive | Adv Search |
Transcriptomic Microarray Analysis Revealed Acetyl-CoA Metabolic Pathway Defect in Male Sterile Line BNS in Wheat (Triticum aestivum)
FU Qing-Yun1, YANG Jing1, SUN Hai-Li1, SU Qing1,2, WEI Xiao1, RU Zhen-Gang1*, LI You-Yong1*
1 School of Life Science and Technology, Henan Institute of Science and Technology/Collaborative Innovation Center of Modern Biological Breeding, Xinxiang 453003, China;
2 College of Life Science, Henan Normal University, Xinxiang 453003, China
Download: PDF (5088 KB)   HTML (1 KB) 
Export: BibTeX | EndNote (RIS)      
Abstract  BNS (Bai-nong sterility) is a nuclear thermo-sensitive male sterile line of common wheat (Triticum aestivum), which plays an important role for the study of hybrid wheat. In order to explore the physiological mechanism of BNS sterility, Affymetrix wheat microarray was used to detect the transcriptomes of BNS male sterility and male fertile in anthers and spike-stalks in the developmental stage of tetrad and mononuclear, and analyzed metabolic networks generated by differential probe genes. The results found that an acetyl-CoA metabolic sub-network could be formed in the profiles of up-regulated genes in fertile anthers, but the sub- network was incompletely in partly sterile anthers, and it was not formed in sterile anthers. The acetyl-CoA sub-metabolic network consisted of 6 nodes, which were acetyl-CoA synthesis and metabolism, acyl-CoA synthesis and metabolism, and thioester synthesis and metabolism, respectively. The nodes of sub-network of acetyl-CoA first linked to that of hormone metabolism, waxy metabolism, amino acid metabolism and linked to ATP synthesis, mitochondrial electron transfer, and so on, then connected with stigma and anther development. Acetyl-CoA was a pivotal substance in cell energy metabolism, so the deficiency of this metabolic pathway directly affected the production of energy carriers such as ATP, thus affecting microspore development. ATP content in anther and flag leaf tissue of BNS sterile and fertile plants were detected, respectively. The results showed the content of ATP in the sterile anther decreased by about 10% compared with fertile anther (P<0.01), and the content of ATP in the sterile flag leaf decreased by 50% compared with fertile flag leaf (P<0.01). The results indicated that there was a shortage of ATP supply in the critical period of microspore development. Thus it was believed that the metabolic defect of acetyl-CoA was an important physiological mechanism for BNS male sterility. The mechanism provides an important clue for revealing the occurrence of BNS infertility.
Key wordsWheat      BNS male sterility      Affymetrix wheat transcriptome microarray      Acetyl-CoA metabolism      ATP content     
Received: 25 February 2022     
ZTFLH:  S512.1  
  Q7  
Corresponding Authors: * liyouyong@163.com; rzgh58@163.com   
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
FU Qing-Yun
YANG Jing
SUN Hai-Li
SU Qing
WEI Xiao
RU Zhen-Gang
LI You-Yong
Cite this article:   
FU Qing-Yun,YANG Jing,SUN Hai-Li, et al. Transcriptomic Microarray Analysis Revealed Acetyl-CoA Metabolic Pathway Defect in Male Sterile Line BNS in Wheat (Triticum aestivum)[J]. 农业生物技术学报, 2023, 31(4): 682-694.
URL:  
http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.04.002     OR     http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I4/682
Copyright © Editorial Board of 农业生物技术学报
Supported by:Beijing Magtech