拟南芥突变体<em>atput3</em>过表达水稻<em>OsPUT1</em>基因对百草枯敏感性的研究

doi:10.3969/j.issn.1674-7968.2023.02.003

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Abstract Polyamines which play an important role in plant growth and development are aliphatic nitrogen-containing bases with low molecular weight and biological activity. Polyamine uptake transporters (PUT) mediate the intracellular and intercellular transport of amino acids, polyamines, paraquat and thiamine. Studies showed that Arabidopsis thaliana polyamine transporter 3 (AtPUT3) mutant atput3 has the ability to resist 0.1 μmol/L paraquat. In order to study the role of rice (Oryza sativa) polyamine transporters (OsPUT) in plant growth and development under paraquat stress, 6 polyamine transporters were identified in the genome of rice by bioinformatics methods. The results showed that OsPUT1 and AtPUT3 proteins which have similar molecular weights and structures were membrane proteins with 12 transmembrane structures and amino acid transport domains, and the N-terminal and C-terminal were on the cytoplasmic side. The overexpression vector pFGC1300-35S-OsPUT1 was constructed by double restriction endonuclease digestion and sequencing, and then transformed into Arabidopsis thaliana atput3 mutant. In the whole life cycle, under short-day condition, the phenotypic differences were not obvious among wild-type (WT), mutant atput3 and overexpression (OE) lines, and there was no significant difference between the length of bolting time and the number of rosette leaves during bolting. There was no significant difference among the 3 germination rate when seeds germinated on 1/2 MS medium (0 μmol/L PQ) for 48 h. However, the seed germination rate of WT and OE lines was significantly lower than that of atput 3 (P<0.05) when seeds germinated on 1/2 MS medium (0.1 μmol/L PQ) for 5 d. After 10 d of seedling growth on 1/2 MS medium (0.1 μmol/L PQ), the phenotype of OE lines returned to WT state. Compared with atput3, the length of taproot of WT and OE lines decreased significantly (P<0.05). It shows that the function of OsPUT1 gene restores the phenotype of atput3 to WT, which proved that rice OsPUT1 protein and Arabidopsis AtPUT3 protein had similar functions. This study provides a reference for further exploring the function of OsPUT1 gene, and provides some enlightenment for further exploring the resistance of rice to PQ.

Key words： Rice Arabidopsis thaliana Polyamine transporter Polyamines Herbicide

Received: 23 March 2022

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Corresponding Authors: *zhangbin27104@163.com

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	ZHANG Bin
	HUANG Ya-Ling
	PENG Ying-Zi
	TENG Jie
	CHEN Wen

Cite this article:

ZHANG Bin,HUANG Ya-Ling,PENG Ying-Zi, et al. Study on the Sensitivity of Arabidopsis thaliana Mutant atput3 Which Overexpressing Rice (Oryza sativa) OsPUT1 Gene to Paraquat[J]. 农业生物技术学报, 2023, 31(2): 242-249.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.02.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I2/242

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