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| Cloning and Expression Analysis of TaGPX8 Gene in Wheat (Triticum aestivum) |
| ZHANG Hua-Dong1,2, LI Ya-Qian1,2, DONG Fei-Yan1,2, DING Fang-Cao3, LIU Meng-Wei2, XU Jia-Sheng1, GAO Chun-Bao1,2, WANG Xiao-Ling1, LIU Yi-Ke2,*, FANG Zheng-Wu1,* |
1 College of Agronomy/MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Yangtze University, Jingzhou 434025, China;
2 Food Crops Institute/Hubei Provincial Key Laboratory of Germplasm Innovation and Genetic Improvement of Food Crops/Wheat Disease Biology Research Station on Central China, Ministry of Agriculture and Rural Affairs, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;
3 Wuhan University of Bioengineering, Wuhan 430403, China |
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Abstract Glutathione peroxidase (GPX), as one of the most important antioxidant enzymes in plants, plays an important role in plant stress response. In the analysis of the transcriptome data of the wheat (Triticum aestivum) GPX gene family, it was found that the TaGPX8 (IWGSC accession No. TraesCS4D02G162000.1) had the characteristics of being induced by stress. In this study, TaGPX8 was cloned, its protein was analyzed by bioinformatics and subcellular localized, and the expression of TaGPX8 in roots and leaves under salt and drought stress were analyzed.The results of gene structure analysis showed that TaGPX8 had a full length of 14 754 bp, including 6 exons and 5 introns, and the full length of CDS was 564 bp, encoding 187 amino acid residues. The relative molecular mass of the encoded protein was 21.330 kD, its theoretical isoelectric point was 6.62, and its average hydrophilicity was -0.664. Promoter sequence analysis found that the promoter sequence of TaGPX8 contained some cis-acting elements involved in hormone response, light response, and stress response. qPCR results revealed that TaGPX8 gene responded to drought and salt stress. Under drought stress, the expression level of TaGPX8 in leaves at 12 h had no significant difference with the control, but was significantly induced at 24 h, its expression level was up regulated, which was 12 times that of the control, and the expression level returned to no significant difference with the control in 48 h. In addition, the expression in roots also increased at 48 h of drought stress treatment. Under salt stress, the expression of TaGPX8 in leaves was inhibited to a certain extent at 12 h of treatment, but the expression level increased at 24 h of treatment, and then recovered to no significant difference with the control at 48 h. However, the expression in the root was suppressed. The yeast transcription activation experiments verified that TaGPX8 did not have transcriptional activation activity. Subcellular localization showed that the TaGPX8 protein was localized in the nucleus and cell membrane. This study provides a reference basis for further research on the function of TaGPX8 gene.
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Received: 18 April 2022
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Corresponding Authors:
*fangzhengwu88@163.com;hbliuyk@foxmail.com
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