Relationship Between AcTPR2 Induced Resistance of Harvested Kiwifruit (Actinidia chinensis) to Botrytis cinerea and Lignin Content
LI Zhe-Xin1,2, REN Yun2, LI Qiang2, JUE Deng-Wei2, TANG Jian-Min2, SONG Hong-Yuan1,*
1 Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China; 2 Chongqing Key Laboratory of Economic Plant Biotechnology/Chongqing Special Plant Industry Collaborative Innovation Center/College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing 402160, China
Abstract Kiwifruit (Actinidia chinensis) is one of the pillar industries for farmers in mountainous areas to fight poverty. Gray mold caused by Botrytis cinerea is easy to cause postharvest corruption of kiwifruit, which seriously restricts the development of kiwifruit industry. In our research group previous study, kiwifruit samples with AcTPR2 gene down-regulated were obtained by virus induced gene silencing (VIGS) method. On this basis, kiwifruit treated with sterile water (WT), transformed with empty vector Tobacco rattle virus (TRV) without AcTPR2 gene, and transgenic kiwifruit (AcTPR2-TRV) that successfully achieved the down-regulation of AcTPR2 gene expression were used as materials, By detecting the lignin content of kiwifruit infected by B. cinerea and the expression of key enzyme genes in lignin biosynthesis pathway, the relationship between AcTPR2 induced resistance of kiwifruit to B. cinerea and the lignin content was explored. The results showed that compared with the control group, a significant increase in lignin content was detected 24~48 h after B. cinerea infection, indicating that the lignin accumulation was involved in the early stage of kiwifruit resistance to B. cinerea infection, and the expression of key enzyme genes in lignin synthesis pathway like phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), coumaric acid 3-hydroxylase (C3H) and cinnamoyl-CoA reductase (CCR) was consistent with the increasing trend of lignin content. The lignin content and the expression of related genes in AcTPR2-TRV fruits increased significantly compared with the control group at 24 h after successful transformation, indicating that kiwifruit with AcTPR2 down-regulated activated the lignin synthesis signal pathway to accelerate lignin biosynthesis and participate in the resistance response of kiwifruit to gray mold (P<0.05). Therefore, the lignification of kiwifruit cell wall regulated by AcTPR2 is related to the resistance response of kiwifruit to B. cinerea, which is one of the mechanisms of disease resistance response in kiwifruit. The results of the present study not only enriched the disease resistance regulation mechanism of kiwifruit, but also provide important gene resource information for disease resistance molecular breeding of kiwifruit in practice.
LI Zhe-Xin,REN Yun,LI Qiang, et al. Relationship Between AcTPR2 Induced Resistance of Harvested Kiwifruit (Actinidia chinensis) to Botrytis cinerea and Lignin Content[J]. 农业生物技术学报, 2023, 31(10): 2048-2057.
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