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| Relationship Between AcTPR2 Induced Resistance of Harvested Kiwifruit (Actinidia chinensis) to Botrytis cinerea and Lignin Content |
| LI Zhe-Xin1,2, REN Yun2, LI Qiang2, JUE Deng-Wei2, TANG Jian-Min2, SONG Hong-Yuan1,* |
1 Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China;
2 Chongqing Key Laboratory of Economic Plant Biotechnology/Chongqing Special Plant Industry Collaborative Innovation Center/College of Landscape Architecture and Life Science/Institute of Special Plants, Chongqing University of Arts and Sciences, Chongqing 402160, China |
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Abstract Kiwifruit (Actinidia chinensis) is one of the pillar industries for farmers in mountainous areas to fight poverty. Gray mold caused by Botrytis cinerea is easy to cause postharvest corruption of kiwifruit, which seriously restricts the development of kiwifruit industry. In our research group previous study, kiwifruit samples with AcTPR2 gene down-regulated were obtained by virus induced gene silencing (VIGS) method. On this basis, kiwifruit treated with sterile water (WT), transformed with empty vector Tobacco rattle virus (TRV) without AcTPR2 gene, and transgenic kiwifruit (AcTPR2-TRV) that successfully achieved the down-regulation of AcTPR2 gene expression were used as materials, By detecting the lignin content of kiwifruit infected by B. cinerea and the expression of key enzyme genes in lignin biosynthesis pathway, the relationship between AcTPR2 induced resistance of kiwifruit to B. cinerea and the lignin content was explored. The results showed that compared with the control group, a significant increase in lignin content was detected 24~48 h after B. cinerea infection, indicating that the lignin accumulation was involved in the early stage of kiwifruit resistance to B. cinerea infection, and the expression of key enzyme genes in lignin synthesis pathway like phenylalanine ammonia lyase (PAL), cinnamate 4-hydroxylase (C4H), coumaric acid 3-hydroxylase (C3H) and cinnamoyl-CoA reductase (CCR) was consistent with the increasing trend of lignin content. The lignin content and the expression of related genes in AcTPR2-TRV fruits increased significantly compared with the control group at 24 h after successful transformation, indicating that kiwifruit with AcTPR2 down-regulated activated the lignin synthesis signal pathway to accelerate lignin biosynthesis and participate in the resistance response of kiwifruit to gray mold (P<0.05). Therefore, the lignification of kiwifruit cell wall regulated by AcTPR2 is related to the resistance response of kiwifruit to B. cinerea, which is one of the mechanisms of disease resistance response in kiwifruit. The results of the present study not only enriched the disease resistance regulation mechanism of kiwifruit, but also provide important gene resource information for disease resistance molecular breeding of kiwifruit in practice.
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Received: 31 July 2022
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Corresponding Authors:
*yuahs@163.com
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[1] 程智慧, 李玉红, 孟焕文, 等. 2006. BTH诱导黄瓜幼苗对霜霉病的抗性与细胞壁HRGP和木质素含量的关系[J]. 中国农业科学, 39(5): 935-940.
(Cheng Z H, Li Y H, Meng H W, 2006. The relationship between BTH-induced resistance to downy mildew in cucumber seedlings and content of HRGP and lignin in cell wall[J]. Scientia Agricultura Sincia, 39(5): 935-940.)
[2] 于明革, 杨洪强, 翟衡. 2003. 植物木质素及其生理学功能[J]. 山东农业大学学报(自然科学版), 34(1): 124-128.
(Yu M G, Li Y H, Meng H W.2003. Lignin and physioligical function in plant[J]. Journal of Shandong Agricultural University (Natural Science), 34(1): 124-128.)
[3] Barnes W, Anderson C.2017. Acetyl bromide soluble lignin (ABSL) assay for total lignin quantification from plant biomass[J]. Bio-Protocal, 7(5): e2149.
[4] Bennett T, Leyser O.2014. Strigolactone signalling: Standing on the shoulders of DWARFs[J]. Current Opinion in Plant Biology, 22: 7-13.
[5] Chen C Y, Séguin-Swartz G.1999. Reaction of wild crucifers to Leptosphaeria maculans, the causal agent of blackleg of crucifers[J]. Canadian Journal of Plant Pathology, 4: 361-367.
[6] Chen H, Cheng Z. Wisniewski M, et al.2015. Ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit[J]. Environmental Science and Pollution Research International, 22(19): 15037-15045.
[7] Guo W, Jin L, Miao Y, et al.2016. An ethylene response-related factor, GbERF1-like, from Gossypium barbadense improves resistance to Verticillium dahliae via activating lignin synthesis[J]. Plant Molecular Biology, 91(3): 305-318.
[8] Hu Q, Min L, Yang X, et al.2017. Laccase GhLac1 modulates broad-spectrum biotic stress tolerance via DAMP-triggered immunity[J]. Plant Physiology, 176(2): 1808-1823.
[9] Kieffer M, Stern Y, Cook H, et al.2006. Analysis of the transcription factor WUSCHEL and its functional homologue in Antirrhinum reveals a potential mechanism for their roles in meristem maintenance[J]. Plant Cell, 18(3): 560-573.
[10] Li X., Longfu Z, Tu L, et al.2011. Lignin metabolism has a central role in the resistance of cotton to the wilt fungus Verticillium dahliae as revealed by RNA-Seq-dependent transcriptional analysis and histochemistry[J]. Journal of Experimental Botany, 62(15): 5607-5621.
[11] Li Z, Lan J B, Liu Y Q, et al.2020. Investigation of the role of AcTPR2 in kiwifruit and its response to Botrytis cinerea infection[J]. BMC Plant Biology, 20: 557.
[12] Liu J, Sui Y, Chen H Z, et al.2018. Proteomic analysis of kiwifruit in response to the postharvest pathogen, Botrytis cinerea[J]. Frontiers in Plant Science, 9: 158.
[13] Long L, Liu J, Gao Y, et al.2019. Flavonoid accumulation in spontaneous cotton mutant results in red coloration and enhanced disease resistance[J]. Plant Physiology and Biochemistry, 143: 40-49.
[14] Ma Q, Zhu H, Qiao M.2018. Contribution of both lignin content and sinapyl monomer to disease resistance in tobacco[J]. Plant Pathology, 67(3): 642-651.
[15] Mohib M, Afnan K, Paran T Z, et al.2017. Beneficial role of citrus fruit polyphenols against hepatic dysfunctions: A review[J]. Journal of Dietary Supplements, 15(2): 223-250.
[16] Navarrete F, Gallei M, Ekornienko A, et al.2022. TOPLESS promotes plant immunity by repressing auxin signaling and is targeted by the fungal effector Naked1[J].Plant Communications, 3(2): 92-111.
[17] Park Y S, Im M H, Gorinstein S.2015. Shelf life extension and antioxidant activity of 'Hayward' kiwi fruit as a result of prestorage conditioning and 1-methylcyclopropene treatment[J]. Journal of Food Science and Technology, 52(5): 2711-2720.
[18] Peer W A.2013. From perception to attenuation: Auxin signaling and responses[J]. Current Opinion in Plant Biology, 16(5): 561-568.
[19] Thines B, Katsir L, Melotto M, et al.2007. JAZ repressor proteins are targets of the SCF(COI1) complex during jasmonate signaling[J]. Nature, 448(7154): 661-665.
[20] Vanholme R, Morreel K, Ralph J, et al.2008. Lignin engineering[J]. Current Opinion in Plant Biology, 11(3): 278-285.
[21] Wang G F, He Y J, Strauch R, et al.2015. Maize homologs of HCT, a key enzyme in lignin biosynthesis, bind the NLR Rp1 proteins to modulate the defense response[J]. Plant Physiology 169 (3): 2230-2043.
[22] Wang R, Estelle M.2014. Diversity and specificity: Auxin perception and signaling through the TIR1/AFB pathway[J]. Current Opinion in Plant Biology, 21: 51-58.
[23] Yan S, Liu Q, Naake T, et al.2021. OsGF14b modulates defense signaling pathways in rice panicle blast response[J]. The Crop Journal, 9(4): 725-738.
[24] Zhu Z, Xu F, Zhang Y, et al.2010. Arabidopsis resistance protein SNC1 activates immune responses through association with a transcriptional corepressor[J]. Proceedings of the National Academy of Sciences of the USA, 107(31): 13960-13965. |
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