牛miR-206潜在靶基因及其组织表达模式分析

doi:10.3969/j.issn.1674-7968.2023.01.009

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Abstract miR-206 is one of the miRNAs previously screened by our group by whole-transcriptome sequencing which showed significant differences in high and low residual feed intake in beef cattle (Bos taurus). Cattle miR-206 was used as a study target to analyze the affinity of miR-206 among species, and the potential target genes of bovine miR-206 were predicted by online software such as TargetScan and miRWalk. GO and KEGG enrichment analysis of the target genes were performed. The expression pattern of miR-206 in cattle tissues was detected by qPCR. The results showed that cattle miR-206 was most closely related to goat (Capra hircus), followed by horse (Equus caballus), and the most distantly related to Xenopus laevis. Among the potential target genes of miR-206 related to muscle development were histone deacetylase 4 (HDAC4), heat shock protein family D member 1 (HSPD1), and fibronectin (FN1). The functional enrichment analysis revealed that the predicted target genes were significantly enriched in MAPK, PI3K-AKT and TNF, which were signaling pathways related to muscle development. qPCR results showed that miR-206 expression was the highest in the leg and longest dorsal muscles of adult cattle and was extremely significant higher than that in other tissues (P<0.01), followed by liver, and the lowest was in the duodenum and subcutaneous. Compared with newborn calves, miR-206 expression was extremely significant down-regulated in subcutaneous fat and up-regulated in dorsal longest muscle and leg muscle (P<0.01) in adult cattle. The results of this study will provide a reference for further investigation of the role of miR-206 in the growth and development of cattle muscle.

Key words： miR-206 Tissue expression Target gene prediction qPCR Enrichment pathway

Received: 01 March 2022

ZTFLH:

S823.8

Corresponding Authors: *kangxl9527@126.com

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	Articles by authors
	ZHANG Yan-Feng
	DING Yan-Ling
	LI Peng
	LI Hao-Ning
	SHI Yuan-Gang
	KANG Xiao-Long

Cite this article:

ZHANG Yan-Feng,DING Yan-Ling,LI Peng, et al. Analysis of Potential Target Genes and Tissue Expression Patterns of Cattle (Bos taurus) miR-206[J]. 农业生物技术学报, 2023, 31(1): 98-104.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.01.009 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I1/98

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