番茄果实成熟过程中GA代谢相关SlGA2ox基因家族受乙烯和ABA调控

doi:10.3969/j.issn.1674-7968.2023.01.003

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (4378 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Fruit ripening is regulated by a variety of plant hormones, such as ethylene, abscisic acid (ABA) and gibberellin (GA), and ripening-related genes. SlGA2ox (SlGA2-oxidase) family are key genes for regulating GA metabolism and play significant role in regulating tomato (Solanum lycopersicum) fruit ripening. Nevertheless, the specific role of SlGA2ox in maturation-related GA metabolism and its interaction with ethylene and ABA remain unclear. In this study, 15 SlGA2ox genes were screened based on the tomato genome database, and the role of SlGA2ox during maturation process was explored through promoter element prediction, gene expression analysis, and the response analysis to GA, ethylene and ABA. The results showed that 12 SlGA2ox genes were closely related to tomato fruit ripening. Among them, SlGA2ox2, SlGA2ox5, SlGA2ox6 and SlGA2ox8 might be regulated by ethylene in the process of mediating GA metabolism, while SlGA2ox4 and SlGA2ox5 might be regulated by ABA at the same time. In addition, SlGA2ox5 was regulated by both ABA and ethylene signals, so it might be a key gene in the fruit ripening which was regulated jointly by the 3 hormones. The present study provides basic material for in-depth exploration of the genes related to tomato fruit ripening regulation, and provides important clues for exploring the molecular mechanism of tomato fruit ripening regulation.

Key words： Solanum lycopersicum Fruit ripening SlGA2-oxidase (SlGA2ox) Gibberellin (GA) Ethylene Abscisic acid (ABA)

Received: 10 May 2022

ZTFLH:

S641.2

Corresponding Authors: *zoujian@cwnu.edu.cn

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHEN Xi-Tong
	YANG Jun
	HE Zhuo-Yuan
	ZENG Wen-Jing
	ZHOU Jia-Yan
	WANG Qian
	ZOU Jian

Cite this article:

CHEN Xi-Tong,YANG Jun,HE Zhuo-Yuan, et al. GA Metabolism-related Gene Family SlGA2ox is Regulated by Ethylene and ABA During Tomato (Solanum lycopersicum) Fruit Ripening[J]. 农业生物技术学报, 2023, 31(1): 25-35.

URL:

http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2023.01.003 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2023/V31/I1/25

[1] Centeno D C, Osorio S, Nunes-Nesi A, et al.2011. Malate plays a crucial role in starch metabolism, ripening, and soluble solid content of tomato fruit and affects postharvest softening[J]. Plant Cell, 23(1): 162-184.
[2] Chen S, Wang X, Zhang L, et al.2016. Identification and characterization of tomato gibberellin 2-oxidases (GA2oxs) and effects of fruit-specific SlGA2ox1 overexpression on fruit and seed growth and development[J]. Horticulture Research, 3: 16059.
[3] Ding Q, Wang F, Xue J, et al.2020. Identification and expression analysis of hormone biosynthetic and metabolism genes in the 2OGD family for identifying genes that may be involved in tomato fruit ripening[J]. International Journal of Molecular Sciences, 21(15): 5344.
[4] El-Sharkawy I, Sherif S, El Kayal W, et al.2014. Characterization of gibberellin-signalling elements during plum fruit ontogeny defines the essentiality of gibberellin in fruit development[J]. Plant Molecular Biology, 84(4): 399-413.
[5] Gao X T, Wu M H, Sun D, et al.2020. Effects of gibberellic acid (GA₃) application before anthesis on rachis elongation and berry quality and aroma and flavour compounds in Vitis vinifera L. 'Cabernet Franc' and 'Cabernet Sauvignon' grapes[J]. Journal of the Science of Food and Agriculture, 100(9): 3729-3740.
[6] García-Hurtado N, Carrera E, Ruiz-Rivero O, et al.2012. The characterization of transgenic tomato overexpressing gibberellin 20-oxidase reveals induction of parthenocarpic fruit growth, higher yield, and alteration of the gibberellin biosynthetic pathway[J]. Journal of Experimental Botany, 63(16): 5803-5813.
[7] Hedden P.2020. The current status of research on gibberellin biosynthesis[J]. Plant and Cell Physiology, 61(11): 1832-1849.
[8] Kawai Y, Ono E, Mizutani M.2014. Evolution and diversity of the 2-oxoglutarate-dependent dioxygenase superfamily in plants[J]. The Plant Journal, 78(2): 328-343.
[9] Klee H J, Giovannoni J J.2011. Genetics and control of tomato fruit ripening and quality attributes[J]. Annual Review of Genetics, 45: 41-59.
[10] Kou X, Yang S, Chai L, et al.2021. Abscisic acid and fruit ripening: Multifaceted analysis of the effect of abscisic acid on fleshy fruit ripening[J]. Scientia Horticulturae, 281: 109999.
[11] Kuhn N, Ponce C, Arellano M, et al.2020. Gibberellic acid modifies the transcript abundance of ABA pathway orthologs and modulates sweet cherry (Prunus avium) fruit ripening in early-and mid-season varieties[J]. Plants, 9(12): 1796.
[12] Løvdal T, Lillo C.2009. Reference gene selection for quantitative real-time PCR normalization in tomato subjected to nitrogen, cold, and light stress[J]. Analytical Biochemistry, 387(2): 238-242.
[13] Li H, Wu H, Qi Q, et al.2019. Gibberellins play a role in regulating tomato fruit ripening[J]. Plant and Cell Physiology, 60(7): 1619-1629.
[14] Li L, Zhang W, Zhang L, et al.2015. Transcriptomic insights into antagonistic effects of gibberellin and abscisic acid on petal growth in Gerbera hybrida[J]. Frontiers in Plant Science, 6: 168.
[15] Li R, Sun S, Wang H, et al.2020. FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato[J]. Nature Communications, 11(1): 1-12.
[16] Liao X, Li M, Liu B, et al.2018. Interlinked regulatory loops of ABA catabolism and biosynthesis coordinate fruit growth and ripening in woodland strawberry[J]. Proceedings of the National Academy of Sciences of the USA, 115(49): E11542-E11550.
[17] Mou W, Li D, Luo Z, et al.2015. Transcriptomic analysis reveals possible influences of ABA on secondary metabolism of pigments, flavonoids and antioxidants in tomato fruit during ripening[J]. PLOS ONE, 10(6): e0129598.
[18] Nelson S K, Steber C M.2016. Gibberellin hormone signal perception: Down-regulating DELLA repressors of plant growth and development[J]. Annual Plant Reviews, 49: 153-188.
[19] Qin H, Pandey B K, Li Y, et al.2022. Orchestration of ethylene and gibberellin signals determines primary root elongation in rice[J]. Plant Cell, 34(4): 1273-1288.
[20] Ribeiro D M, Araújo W L, Fernie A R, et al.2012. Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis[J]. Journal of Experimental Botany, 63(7): 2769-2786.
[21] Shinozaki Y, Hao S, Kojima M, et al.2015. Ethylene suppresses tomato (Solanum lycopersicum) fruit set through modification of gibberellin metabolism[J]. The Plant Journal, 83(2): 237-251.
[22] Shu K, Chen Q, Wu Y, et al.2016. ABI4 mediates antagonistic effects of abscisic acid and gibberellins at transcript and protein levels[J]. The Plant Journal, 85(3): 348-361.
[23] Vicente A R, Saladie M, Rose J K, et al.2007. The linkage between cell wall metabolism and fruit softening: Looking to the future[J]. Journal of the Science of Food and Agriculture, 87(8): 1435-1448.
[24] Zou J, Chen J, Tang N, et al.2018. Transcriptome analysis of aroma volatile metabolism change in tomato (Solanum lycopersicum) fruit under different storage temperatures and 1-MCP treatment[J]. Postharvest Biology and Technology, 135: 57-67.
[25] Zou J, Li N, Hu N, et al.2022. Co-silencing of ABA receptors (SlRCAR) reveals interactions between ABA and ethylene signaling during tomato fruit ripening[J]. Horticulture Research, 9: uhac057.
[26] Zuo J, Wang Y, Zhu B, et al.2018. Comparative analysis of DNA methylation reveals specific regulations on ethylene pathway in tomato fruit[J]. Genes, 9(5): 266.