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| Cloning of Commd9 Gene in Amur ide (Leuciscus waleckii) and Its Expression Analysis Under Alkalinity Stress |
| LUO Liang1,2, CHANG Yu-Mei2, SUN Bo2, ZHANG Li-Min1, CHEN Jiong1,*, LIANG Li-Qun2,* |
1 Laboratory of Biochemistry and Molecular Biology/School of Marine Science, Ningbo University, Ningbo 315832, China;
2 Key Laboratory of Fish Stress Resistance Breeding and Germplasm Characteristics on Special Habitats in Heilongjiang Province/Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China |
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Abstract The copper metabolism Murr1 domain (COMMD) protein family is an important biologic part of the nuclear factor-κB (NF-κB) pathway, sodium transport, copper regulation, and adaptive regulation in hypoxia. Commd9 cDNA in high-alkaline-tolerant fish, Leuciscus waleckii, was cloned firstly and given the name LwCommd9 (GenBank No. MT013199) by rapid-amplification of cDNA ends (RACE) to find out what Commd9 does in alkaline environment. The results showed that the full-length cDNA sequence of the Commd9 gene was 938 bp in L. waleckii, which had 5 bp of 5' non-coding region, 339 bp of 3' non-coding region, and 594 bp of the ORF fragment could code for 197 amino acids that had a predicted molecular weight of about 21.79 kD and a theoretical isoelectric point of 5.63. Phylogenetic analysis revealed that the amino acid sequence of LwCommd9 showed high similarity with carp, particularly with Anabarilius grahami up to 93.37%. qPCR showed that LwCommd9 were expressed in virous organs, such as significant increase in liver and kidney (P<0.05). In addition, LwCommd9 expression in liver was significantly down regulated (P<0.01) in alkaline water species (alkali-water, AW), but that was highly up regulated (P<0.01) in freshwater species (FW) and showed in kidney with the same trend. The above results indicate that the Commd9 gene in L. waleckii might play a critial role in the process of high-alkaline adaptation, and the in-depth mechanisms related to this pathway can be explored deeply.
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Received: 15 March 2022
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Corresponding Authors:
*jchen1975@163.com;liangliqun@hrfri.ac.cn
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