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| Association Between Polymorphisms in B4GALNT2 Gene and Litter Size in Mongolia Sheep and Ujimqin Sheep (Ovis aries) |
| GAO Yi-Jie1,*, WANG Jin2,*, CHAO Lu-Meng3, CANG Ming1, YU Hai-Quan1, WANG Jian-Guo1, BAO Si-Qin1, LIU Yong-Bin4, ZHANG Wen-Guang5, MA Qing2,**, TONG Bin1,** |
1 State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock/School of Life Sciences, Inner Mongolia University, Hohhot 010071, China;
2 Institute of Animal Sciences, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750001, China;
3 Agricultural and Animal Husbandry Technology Extension Center of Xilinhot City, Xilinhot 026000, China;
4 Animal Husbandry Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry, Hohhot 010031, China;
5 College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010071, China |
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Abstract Litter size is an important economic trait in sheep (Ovis aries) breeding, which determines the production efficiency of mutton sheep industry. Studies have shown that the g.36938224A>T SNP in β-1,4-N-acetyl-galactosaminyl transferase 2 gene (B4GALNT2) was a major candidate marker associated with the ovulation rate of French Lacaune sheep breed, therefore, the B4GALNT2 gene was considered as a new candidate gene for fecundity in sheep. The aims of this study were to confirm the association between the known SNPs in the B4GALNT2 and litter size of Mongolia and Ujimqin sheep breeds, and identify novel breed-specific variants of Mongolia sheep as well as perform the association study with litter size of Mongolia and Ujimqin sheep breeds. A total of 480 sheep were used in this study including 250 Mongolia sheep, 110 Ujimqin sheep, 30 Hulunbuir sheep, 30 Tan sheep, 30 Small-tailed Han sheep, and 30 Hu sheep. Then, direct sequencing was used to identify novel polymorphism and detect the g.25929884A>T SNP of B4GALNT2 gene (GenBank No. NC_040262.1), the MassARRAY technology was used to genotype the g.25921552C>T and g.25935026C>T SNPs of B4GALNT2 gene and c.*803A>G SNP of distal-less homeobox 3 (DLX3) gene in 480 samples of Mongolia sheep, Ujimqin sheep, Hulunbuir sheep, Tan sheep, Small-tailed Han sheep and Hu sheep. Therefore, association studies were performed between the novel variants and litter size in Mongolia and Ujimqin sheep breeds. The results showed that the g.25929884A>T SNP of B4GALNT2 and the c.*803A>G SNP of DLX3 were absent in any sheep breed. The g.25921552C>T and g.25935026C>T SNPs of B4GALNT2 gene were not associated with litter size of Mongolia and Ujimqin sheep. Furthermore, 8 novel SNPs were found in B4GALNT2 gene, among them, the g.25929637G>A (P<0.05), g.25929679T>C (P<0.01), g.25929819A>G (P<0.01), and g.25929965A>T (P<0.05) SNPs were associated with litter size of Mongolia sheep. Except for g.25921552C>T site in Tan sheep, the other 9 polymorphic loci were under Hardy-Weinberg equilibrium. According to PIC standard, all SNPs presented low and moderate polymorphism in 6 sheep breeds. The presnet study might facilitate effective marker-assisted selection to increase litter size in Mongolia sheep, and provide scientific basis for genetic improvement of sheep.
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Received: 05 October 2021
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Corresponding Authors:
**maqing1973@126.com; tongbin87@163.com
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| About author:: * These authors contributed equally to this work |
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