|
|
|
| Expression Characteristics and Correlation Analysis of HDAC6 and AREG of Different Developmental Stages Follicles in Tan Sheep Ovarian (Ovis aries) |
| FAN Shan-Shan1,2, LIU Yu-Jun1,2, XU Ya-Xiu1,2, YANG Yi1,2, LIU Xin-Feng1,2,*, WANG Chao3,* |
1 Key laboratory of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan 750021, China;
2 College of Life Sciences, Ningxia University, Yinchuan 750021, China;
3 State Key Laboratory of Agricultural Biotechnology, College of Biology, China Agricultural University, Beijing 100193, China |
|
|
|
|
Abstract Histone deacetylase 6 (HDAC6) is an important epigenetic modification protein and amphiregulin (AREG) is an important epithelial growth factor-like (EGF-like). HDAC6 and AREG play important roles in regulating mammalian reproduction. This study aims to explore the expression rules of HDAC6 and AREG in ovarian follicles with different development stages of Tan sheep (Ovis aries) and analyze their correlation in follicle development, in this study, the ovaries of Tan sheep were collected for detecting the localization and expression patterns of HDAC6 and AREG in ovarian follicles with different development stages of Tan sheep by immunohistochemistry, immunofluorescence and Western blot methods, and the correlation between HDAC6 and AREG in follicular development was analyzed by Pearson method. The results showed that HDAC6 was expressed in both granulosa cells and oocytes of ovarian follicles at different developmental stages of Tan sheep, and was mainly expressed in the cytoplasm of granulosa cells and oocytes. With the development of follicles, the expression level of HDAC6 increased first and then decreased in follicles with different development stages, and the expression level of HDAC6 in follicles larger than 6 mm in diameter was significantly lower than that in follicles less than 2 mm in diameter. AREG was mainly expressed in granulosa cells of different sizes of ovarian follicles of Tan sheep, and was mainly expressed in granulosa cytoplasm. With the development of follicles, the expression level of AREG was gradually increased in follicles with different development stages, and the expression level of AREG in follicles with diameters greater than 6 mm was significantly higher than that in follicles with diameters less than 2 mm. Furthermore, the correlation analysis found that the expression level of HDAC6 was negatively correlated with that of AREG in follicles with different diameters of Tan sheep. The expression level of HDAC6 in follicles with diameters of less than 2 mm was moderately negatively correlated with that of AREG, and the expression level of HDAC6 in follicles with diameters of 2~6 mm was strongly negatively correlated. The expression level of AREG was negatively correlated with HDAC6 in follicles larger than 6 mm with diameter. The results showed that HDAC6 and AREG were expressed in ovarian follicles with different development stages of Tan sheep, and the expression patterns of HDAC6 and AREG were negatively correlated in general, suggesting that HDAC6 and AREG may be involved in ovarian follicle development of Tan sheep through negative correlation regulation. This study provides an important reference for further exploring the expression relationship of HDAC6 and AREG in granulosa cells and oocytes at different development stages of Tan sheep ovarian follicles and their effects on follicular development and oocyte maturation, and possible mechanisms.
|
|
Received: 22 November 2021
|
|
|
|
Corresponding Authors:
*wangcam@126.com; liu2019074@nxu.edu.cn
|
|
|
|
[1] 隋立艳. 2020. HDAC6和HDAC11调节的组蛋白表观遗传学修饰在小鼠卵母细胞成熟中的作用及机制研究[D]. 博士学位论文, 吉林大学, 导师: 李子义, pp. 21-33.
(Sui L Y.2020. Effects and mechanism of histone epigenetic modification regulated by HDAC6 and HDAC11 in mouse oocyte maturation[D]. Thesis for Ph.D., Jilin University, supervisor: Li Z Y, pp. 21-33.)
[2] 郭延华, 周平, 石国庆, 等. 2012. 绵羊不同直径卵泡卵母细胞特征及染色体形态分析[J]. 新疆农业科学, 49(07):1307-1314.
(Guo Y H, Zhou P, Shi G Q, et al.2012. Characteristics and chromosome morphology of sheep oocytes with different diameter follicles[J]. Xinjiang Agricultural Sciences, 49(07): 1307-1314.)
[3] 马鸿程, 熊显荣, 海卓, 等. 2021. 去甲基化酶KDM4B在牦牛卵泡发育过程中的表达[J]. 中国兽医学报, 41(05):1016-1022.
(Ma H C, Xiong X R, Hai Z, et al.2021. Expression of demethylase KDM4B during follicular development in yaks[J]. Chinese Journal of Veterinary Medicine, 41(05): 1016-1022.)
[4] 马小明, 李颖康. 2017. 宁夏羊产业发展现状[J]. 黑龙江畜牧兽医, (12): 67-70.
(Ma X M, Li Y K. 2017. Development status of sheep industry in Ningxia[J]. Hei long jiang Animal Science and Veterinary Medicine, (12): 67-70.)
[5] 李昊, 葛翠翠, 冯帆, 等. 2019. 脂肪酸在育肥滩羊、小尾寒羊及滩寒杂交羊肌肉组织中的含量特征[J]. 食品研究与开发, 40(3): 157-161.
(Li H, Ge C C, Feng F, et al.2019. Fatty acids in longissimus dorsi from fattening tan sheep, small fat-tail sheep and tan han hynrid sheep[J]. Food Research and Development, 40(03): 157-161.)
[6] 秦文昌, 殷实, 李泽沛, 等. 2020. 牦牛HDAC8基因特征及其在组织和卵母细胞成熟过程中的表达分析[J]. 华北农学报, 35(02): 210-216.
(Qin W C, Yin S, Li Z P, et al.2020. The characteristic of Yak HDAC8 gene and analysis of their expression in different tissues and during the oocyte maturation[J]. Acta Agriculturae Boreali-Sinica, 35(02): 210-216.)
[7] 王兆琛, 赵勇超, 杜炜, 等. 2020. AREG对绵羊小腔卵泡卵母细胞体外成熟的影响[J]. 畜牧兽医学报, 51(06):1238-1247.
(Wang Z C, Zhao Y C, Du W, et al.2020. Effect of AREG on in vitro maturation of small cavitary follicular oocytes of sheep[J]. Chinese Journal of Animal Science and Veterinary Medicine, 51(06): 1238-1247.)
[8] 王中伟. 2011. HDAC1基因在牛卵母细胞和早期克隆胚胎发育中的作用[D]. 博士学位论文, 吉林大学, 导师: 李子义, pp. 45-61.
(Wang Z W.2011. Function of HDAC1 gene during the development of bovine oocytes and cloned embryos[D]. Thesis for Ph.D., Jilin University, Supervisor: Li Z Y, pp. 45-61.)
[9] 魏强强. 2017. 宁夏滩羊繁殖生产试验及影响滩羊群体繁殖效率的因素探讨[D]. 硕士学位论文, 西北农林科技大学, 导师: 马保华, 牛文智, pp. 16-24.
(Wei Q Q.2017.The breeding production trail and the affecting of breeding efficiency in Ning xia Tan sheep population[D]. Thesis for M.S., Northwest A＆F University, Supervisor: Ma B H, Niu W Z, pp. 16-24.)
[10] 赵金宇. 2013. 宁夏滩羊早期生长发育规律和生产性能研究[D]. 硕士学位论文, 西北农林科技大学, 导师: 罗军, pp. 21-23.
(Zhao J Y.2013. Study on the growth-development and production of Tan Sheep in Ningxia[D]. Thesis for M.S., Northwest A＆F University, Supervisor: Luo J, pp. 21-23.)
[11] Chen S, Owens G C, Makarenkova H, et al.2010. HDAC6 regulates mitochondrial transport in hippocampal neurons[J]. PLOS ONE, 5(5): e10848.
[12] Downs S, Daniel S, Eppig J.1988. Induction of maturation in cumulus cell-enclosed mouse oocytes by follicle-stimulating hormone and epidermal growth factor: Evidence for a positive stimulus of somatic cell origin[J]. The Journal of Experimental Zoology, 245: 86-96.
[13] Guidi A, Saccoccia F, Gennari N, et al.2018. Identification of novel multi-stage histone deacetylase (HDAC) inhibitors that impair Schistosoma mansoni viability and egg production[J]. Parasites & Vectors, 11(1): 668.
[14] Gu L, Li X, Liu X, et al.2019. HDAC3 inhibition disrupts the assembly of meiotic apparatus during porcine oocyte maturation[J]. Journal of Cellular Physiology, 234(7):10178-10183.
[15] Kalinski A, Kar A, Craver J, et al.2019. Deacetylation of Miro1 by HDAC6 blocks mitochondrial transport and mediates axon growth inhibition[J]. The Journal of Cell Biology, 218(6): 1871-1890.
[16] Legoff L, Dali O, De La Mata Santaella E, et al.2021. Histone deacetylase inhibition leads to regulatory histone mark alterations and impairs meiosis in oocytes[J]. Epigenetics Chromatin, 14(1): 39.
[17] Ling L, Hu F, Ying X, et al.2018. HDAC6 inhibition disrupts maturational progression and meiotic apparatus assembly in mouse oocytes[J]. Cell Cycle, 17(5): 550-556.
[18] Matoba S, Liu Y, Lu F, et al.2014. Embryonic development following somatic cell nuclear transfer impeded by persisting histone methylation[J]. Cell, 159(4): 884-895.
[19] Morelli G, Even A, Gladwyn-Ng I, et al.2018. p27Kip1 modulates axonal transport by regulating α-tubulin acetyltransferase 1 stability[J]. Cell Reports, 23(8): 2429-2442.
[20] Park J Y, Su Y Q, Ariga M, et al.2004. EGF-like growth factors as mediators of LH action in the ovulatory follicle[J]. Science, 303(5658): 682-684.
[21] Procházka R, Petlach M, Nagyová E, et al.2011. Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in vitro: Comparison with gonadotropins[J]. Reproduction, 141(4): 425-435.
[22] Pulya S, Amin S A, Adhikari N, et al.2021. HDAC6 as privileged target in drug discovery: A perspective[J]. Pharmacological Research, 163: 105274.
[23] Richani D, Gilchrist R B.2018. The epidermal growth factor network: Role in oocyte growth, maturation and developmental competence[J]. Human Reproduction Update, 24(1): 1-14.
[24] Sakaguchi K, Yanagawa Y, Yoshioka K, et al.2019. Relationships between the antral follicle count, steroidogenesis, and secretion of follicle-stimulating hormone and anti-Müllerian hormone during follicular growth in cattle[J]. Reproductive Biology and Endocrinology, 17(1): 88.
[25] Shen S, Kozikowski A P.2020. A patent review of histone deacetylase 6 inhibitors in neurodegenerative diseases (2014-2019)[J]. Expert Opinion on Therapeutic Patents, 30(2): 121-136.
[26] Shimada M, Umehara T, Hoshino Y.2016. Roles of epidermal growth factor (EGF)-like factor in the ovulation process[J]. Reproductive Medicine and Biology, 15(4): 201-216.
[27] Shimizu T.2016. Molecular and cellular mechanisms for the regulation of ovarian follicular function in cows[J]. The Journal of Reproduction and Development, 62(4): 323-329.
[28] Sui L, Huang R, Yu H, et al.2020. Inhibition of HDAC6 by tubastatin A disrupts mouse oocyte meiosis via regulating histone modifications and mRNA expression[J]. Journal of Cellular Physiology, 235(10): 7030-7042.
[29] Sun J, Liu Q, Lv L, et al.2021. HDAC6 is involved in the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in Pig[J]. Reproductive Sciences, 28(9): 2630-2640.
[30] Wang H, Cai H, Wang X, et al.2019. HDAC3 maintains oocyte meiosis arrest by repressing amphiregulin expression before the LH surge[J]. Nature Communications, 10(1): 5719.
[31] Zhang T, He M, Zhao L, et al.2021. HDAC6 regulates primordial follicle activation through mTOR signaling pathway[J]. Cell Death & Disease, 12(6): 559. |
|
|
|
