Physiological Changes and Related Gene Expression Analysis of Sesuvium portulacastrum Under Salt Stress
LI Yu-Xin1, LUO Xiu-Li1, ZHANG Ting-Ting1, KANG Yu-Qian1, WANG Peng1, JIANG Xing-Yu2,ZHOU Yang1*
1 School of Horticulture/Key Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, Hainan University, Haikou 570228, China;
2 School of Tropical Crops/Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops, Hainan University, Haikou 570228, China
Abstract Salt stress is one of the main abiotic stresses that restrict plant growth and development. In this study, the halophyte Sesuvium portulacastrum seedlings were treated with 400 and 800 mmol/L NaCl for 0, 6,12, 24, 48 and 72 h, and the changes of physiological indexes in roots and leaves and the expression level of salt-related genes were analyzed. The results showed that Sesuvium portulacastrum seedlings withered at the beginning of stress, while the growth gradually stabilized with the extension of stress time when treated with 400 mmol/L NaCl. While the seedlings wilted and died gradually with the extension of stress time under 800 mmol/L NaCl. The activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and peroxidase (POD) in leaves and roots all increased significantly under 12 h treatment. The accumulation of soluble sugar and proline increased obviously, and then the enzyme activity and osmotic regulation substances contents decreased. The relative conductivity increased first and then decreased. Four salt-tolerant genes were selected for real-time fluorescence quantitative verification. The expression levels of salt-related genes, plasma membrane Na+/H+ antiporter gene salt overly sensitive 1 (SOS1), protein kinase gene CBL-interaccting protein kinase 8 (CIPK8), Calcineurin B-like 10 (CBL10) and plasma membrane H+-ATPase gene (AHA1), were up- regulated in the roots and leaves under salt stress. The expression levels of SpSOS1, SpCIPK8, SpCBL10 and SpAHA1 genes under 800 mmol/L NaCl reached the highest at 12 h after treatment, and the expressions were 6.80, 124.32, 25.93 and 9.52 times of those under 400 mmol/L NaCl at the same time. This study provides a theoretical basis for the study of physiological indexes and related gene expression regulation of plants under salt stress.
LI Yu-Xin,LUO Xiu-Li,ZHANG Ting-Ting, et al. Physiological Changes and Related Gene Expression Analysis of Sesuvium portulacastrum Under Salt Stress[J]. 农业生物技术学报, 2022, 30(7): 1279-1289.
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