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| Preliminary Analysis on the Functions of SLC25A4 Gene in Anthocyanin Synthesis of Guizi Wheat (Triticum aestivum) |
| LI Lu-Hua1,2, XIONG Fu-Min1,2, AN Chang1,2, PENG Ya-Shu1,2, REN Ming-Jian1,2, XU Ru-Hong1,2* |
1 College of Agriculture, Guizhou University, Guiyang 550025, China;
2 Guizhou Sub-Center of National Wheat Improvement Center, Guiyang |
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Abstract The solute carrier family 25 (SLC25) members participate in regulation of anthocyanin biosynthesis. The SLC25 family member SLC25A4 is adenine nucleotide translocase 1 (ANT1), which plays important roles in growth and development, and response to stresses of plant. To enrich the research of SLC25A4 in wheat (Triticum aestivum) and explore biological functions of SLC25A4 in the regulation of anthocyanin biosynthesis, the expression of SLC25A4 was analyzed based on transcriptome sequence results at different developmental stages of 'Guizi 1' wheat grains and was further verified by qPCR. The results demonstrated that the expression of TaSLC25A4-7A, TaSLC25A4-7B and TaSLC25A4-7D1 genes was significantly higher at 25 days post anthesis (DPA) and 35 DPA than that at 10 DPA (P<0.05), particularly qPCR analysis showed that the dynamic change of TaSLC25A4-7A gene expression was consistent with that of anthocyanin accumulation. Furthermore, TaSLC25A4-7A gene overexpression transgenic tobacco (Nicotiana tabacum) lines were obtained, qPCR results showed that the expression level of anthocyanin synthesis related genes (NtDFR, Nt4CL, NtCHI and NtF3H) was significantly higher in transgenic lines than that in wild type (P<0.05), which demonstrated that TaSLC25A4-7A involved in the biological process of regulating anthocyanin synthesis. The results of carbonyl cyanide m-chlorophenylhydrazone (CCCP) treatment to transgenic lines and wild type demonstrated that the TaSLC25A4-7A overexpression transgenic lines were more sensitive to CCCP. The above results demonstrated that TaSLC25A4 gene (especially TaSLC25A4-7A) is involved in anthocyanin synthesis, which provides reference for further exploring the biological function of the TaSLC25A4 gene in anthocyanin accumulation and stresses response of wheat.
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Corresponding Authors:
* xrhgz@163.com
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