Studies on Resistance of Transgenic cry1A. 301 Maize (Zea mays) to Major Lepidopteran Pests
YANG Xiao-Yan1, WANG Yun-He2, WENG Jian-Feng3, WU Hong1, HAN Yao1, LEI Kai-Rong1, XIE Shu-Zhang1*
1 Chongqing Key Laboratory of Adversity Agriculture Research, Institute of Biotechnology, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China;
2 College of Agriculture, Northeast Agricultural University, Harbin 150030, China;
3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract Ostrinia furnacalis, Mythimna separata and Helicoverpa armigera are the main lepidopteran pests to corn. In order to assess the resistance of transgenic cry1A. 301 maize to these insect pests, two events of CM8201 and CM8204 were evaluated through in vitro tissues laboratory bioassay and artificial infestation in field trials. The results of laboratory bioassay showed that the larval mortality rate of O. furnacalis, M. separate and H. armigera was above 95% when the larvae fed on the heart leaves, silks and grains of CM8201 and CM8204 after 5 d. Hence, the heart leaves, silks and grains of CM8201 and CM8204 showed high resistance to these pests. The results of insect identification on field showed that at whorl stage, as far as the resistance to O. furnacalis be concerned, the mean values of feeding grade of CM8201 and CM8204 were 1.17 and 1.18, respectively. The pest grade was 1. The resistance level was high. As far as the resistance to M. separate be concerned, the mean values of feeding grade of CM8201 and CM8204 were 1.38 and 1.35, respectively, and the resistance type was high. At silking stage, as far as the resistance to O. furnacalis be concerned, the average damage level of female ear of CM8201 and CM8204 was 1.27 and 1.30, respectively. As far as the resistance to H. armigera be concerned, the average damage level of female ear was 0.15 and 0.20, respectively. In conclusion, the transgenic insect-resistant maize CM8201 and CM8204 showed good insecticidal effects against 3 major maize lepidopteran pests at all stages of maize growth. This study provides material basis for the breeding of new insect-resistant transgenic maize varieties.
YANG Xiao-Yan,WANG Yun-He,WENG Jian-Feng, et al. Studies on Resistance of Transgenic cry1A. 301 Maize (Zea mays) to Major Lepidopteran Pests[J]. 农业生物技术学报, 2022, 30(7): 1247-1256.
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