Abstract Nuclear Factor Y (NF-Y) is a transcription factor widely found in eukaryotes and involved in plant growth and development as well as biotic and abiotic stress responses. Phoebe bournei is an important economic timber tree in South China, but the growth and accumulation of wood are seriously affected by the lack of water. Exploration of drought-resistance response factors will provide support for improving the drought resistance of P. bournei. In this study, the whole genome identification and bioinformatics analysis of the NF-Y gene family in P. bournei were conducted, and the tissue expression specificity and expression pattern of PbNF-Ys under drought stress were analyzed by qPCR. The results showed that a total of 40 PbNF-Ys were identified in the whole genome of P. bournei, which were divided into 3 subfamilies and unevenly distributed on 11 chromosomes. The molecular weights of PbNF-Ys protein ranged from 10 774.45 (PbNF-YB11) to 39 576.26 D (PbNF-YA2), and all proteins were hydrophilic. The analysis of promoter elements showed that there were hormone response elements such as auxin, gibberellin and methyl jasmonate, light response elements, growth- and development-related elements and drought stress-related elements. Tissue expression analysis of PbNF-Ys in the root, stem, and leaf was carried out by qPCR. The result showed that PbNF-Ys presents tissue-specific expression patterns. Expression analysis of drought stress treatments showed that 17 PbNF-Ys were up-regulated induced by drought stress, 16 ones were down-regulated, and 7 ones presented no significant expression change. This study provides the reference basis for further study on the biological function and evolution of NF-Y gene family in P. bournei.
HAN Shuang,HAN Xiao,LI Yi-Bei, et al. Identification of NF-Y Gene Family and Expression Analysis in Response to Drought Stress in Phoebe bournei[J]. 农业生物技术学报, 2022, 30(6): 1112-1127.
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