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| Cloning and Bioinformatics Analysis of GbUGT73C1 Gene Responding to Fusarium wilt Resistance in Gossypium barbadense |
| DENG Xiao-Juan*, LU Xiao-Shuang, ZHANG Meng-Jie, HAN Wan-Li, CHEN Quan-Jia, QU Yan-Ying |
| College of Agriculture, Xinjiang Agricultural University , Urumqi 830052, China |
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Abstract Fusarium wilt seriously endangers the growth and development of sea islands cotton (Gossypium barbadense). Previous study based on the transcriptome data of sea island cotton inoculated with Fusarium oxysporum, the resistance-related gene uridine diphosphate glycosyltransferase 73C1 (GbUGT73C1) was screened and identified. In this study, the full length of the GbUGT73C1 gene CDS was found through the sea island cotton genome, and the gene was cloned from '06-146' with high resistance to Fusarium wilt. The cloned nucleic acid and amino acid sequences were performed with bioinformatics analysis, and the gene expression was analyzed after inoculation with disease resistance and susceptible material. The results showed that the full length of CDS of GbUGT73C1 was 1 486 bp, a total of 476 amino acids were encoded. The coding protein included hydrophobic, fat-soluble and unstable protein, without transmembrane structure and signal peptide. With glycocyltransferase-B (GT-B) structure, it belonged to the glycosyltransferase GT-B superfamily. GbUGT73C1 protein was predicted to be localized in the cytoplasmic. The prediction of molecular phylogenetic relationship was closest to that of G. barbadense and G. raimondii. GbUGT73C1 was expressed rapidly in disease-resistant material, and the expression level was extremely significantly higher than that in disease-susceptible material (P<0.01), indicated that resistant '06-146' resisted Fusarium wilt infection by expressing GbUGT73C1 gene more rapidly and in a higher amount .This study lays a foundation for further investigation on the mechanism of GbUGT73C1 response to sea island cotton's resistance to Fusarium wilt, and provides important gene resources for cotton disease-resistance molecular breeding.
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Received: 05 July 2021
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Corresponding Authors:
* dengxj007@163.com
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