|
|
|
| A Quick Molecular Identification for the Species Sources of Animal Fibres |
| XIANG Hai1, ZHU Piao1, ZHANG Xing1,2, YANG Liu1,2, ZHAO Xing-Bo1,2,* |
1 Guangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding, College of Life Science and Engineering, Foshan University, Foshan 528231, China;
2 College of Animal Science and Technology, China Agricultural University, Beijing 100193, China |
|
|
|
|
Abstract Identification of species origin of animal fibres is important in textile inspection work. In this study, a universal primer set for yak (Bos grunniens), camel (Camelus dromedarius and Camelus bactrianus), sheep (Ovis aries), goat (Capra hircus), cattle (Bos taurus) and pig (Sus scrofa) were designed according to their mitochondrial genomes. Moreover, species-specific primer sets for yak, camel, sheep and goat as well as a universal primer set for goat and sheep were also generated. After that, several DNA extraction methods were compared to find out the most optimal method for animal wool shaft fiber. Then the preservation status of samples were determined rapidly and examined whether it was mixed accurately by sequencing the amplicon of the universal primer set. By combining the species-specific primers amplification and electrophoresis detection, we are able to identify the animal species sources of wool shaft fibers with limit time and cost. In this study, an accurate, rapid and simple molecular identification system was established to distinguish the animal species sources of wool shaft fibers, which provides a technical reference for the quality control of textile products.
|
|
Received: 06 July 2021
|
|
|
|
Corresponding Authors:
*zhxb@cau.edu.cn
|
|
|
|
[1] 高炳生, 张俊峰, 陆素红. 2010. 羊毛(羊绒)/棉织物的染整加工[J]. 印染, 36(3): 34-37.
(Gao B S, Zhang J F, Lu S H.2010. Wet processing of wool (cashmere)/cotton fabric[J]. China Dyeing and Finishing, 36(3): 34-37.)
[2] 郭春花. 2015. 保护山羊绒优质资源迫在眉睫[J]. 纺织服装周刊, 31: 34.
(Guo C H.2015. Extremely urgent to protect the high-quality resources of cashmere[J]. Textile Apparel Weekly, 31: 34.)
[3] 李玲, 刘军. 2008. 鉴别羊绒与羊毛混合物的方法[J]. 黑龙江纺织, 1: 8-10.
(Li L, Liu J.2008. The measuring methods of cashmere and wool compound[J]. Heilongjiang Textile, 1: 8-10.)
[4] 李艳红, 麻岩, 李亚乔. 2012. 羊绒、羊毛纤维鉴别检测方法综述[J]. 中国纤检, (11): 58-61.
(Li Y H, Ma Y, Li Y Q. 2012. A summary of detection method of cashmere, wool fiber identification[J]. China Fiber Inspection, (11): 58-61.)
[5] 林志武. 2000. 用生物芯片法快速鉴别羊绒羊毛的展望[J]. 毛纺科技, (4): 28-29.
(Lin Z W. 2000. Prospect of rapid identification of cashmere wool by biochip method[J]. Wool Textile Journal, (4): 28-29.)
[6] 沈建军, 张金莲, 易洪雷, 等. 2018. 骆马毛与羊绒, 羊驼毛基本性能对比研究[J]. 毛纺科技, 46(6): 1-5.
(Shen J J, Zhang J L, Yi H L, et al.2018. Comparative study on the basic properties of vicuna, cashmere and alpaca fibers[J]. Wool Textile Journal, 46(6): 1-5.)
[7] 沈巍. 2008. 基于图像处理技术检测羊绒/羊毛混纺比[D]. 江南大学.
(Shen W.2008. Measuring the blend ratio of cashmere/wool yarns based on image processing technology[D]. Jiangnan University,)
[8] 张鹏芳, 王利平. 2020. 羊毛/羊绒/莫代尔纤维混纺针织物热湿舒适性能综合评价[J]. 毛纺科技, 48(6): 81-85.
(Zhang P F, Wang L P.2020. Comprehensive evaluation of thermal and moisture comfort performance of wool/cashmere/modal blended knitted fabric[J]. Wool Textile Journal, 48(6): 81-85.)
[9] 赵振, 杨周杰. 2020. 粗纺羊绒/绢丝/TencelTM混纺纱线工艺探讨[J]. 纺织导报, (4): 44-46.
(Zhao Z, Yang Z J. 2020. Discussion on the spinning process of woolen cashmere/spun silk/TencelTM blended yarn[J]. China Textile Leader, (4): 44-46.)
[10] Allafi F, Hossain M S, Lalung J, et al.2020. Advancements in applications of natural wool fiber: Review[J]. Journal of Natural Fibers, 2020: 1745128.
[11] Bai M, Amu G.2019. Study on physical properties of camel cashmere[J]. Journal of Camel Practice and Research, 26(2): 191.
[12] Di Domenico M, Di Giuseppe M, Wicochea Rodriguez J D, et al.2017. Validation of a fast real-time PCR method to detect fraud and mislabeling in milk and dairy products[J]. Journal Dairy Science, 100(1): 106-112.
[13] Guan F, Jin Y T, Zhao J, et al.2018. A PCR method that can be further developed into PCR-RFLP assay for eight animal species identification[J]. Journal of Analytical Methods in Chemistry, 2018: 5890140.
[14] Guo H, Song H, Gan L, et al.2020. Is it feasible to use dyed wool powder as pigment[J]. Advanced Powder Technology, 31(12): 4632-4641.
[15] Ismail S A, Taleb M A, Emran M A, et al.2020. Benign felt-proofing of wool fibers using a keratinolytic thermophilic alkaline protease[J]. Journal of Natural Fibers, 2020: 1848721.
[16] Liu C, Xie C, Liu X.2017. Properties of yak wool in comparison to cashmere and camel hairs[J]. Journal of Natural Fibers, 15(2): 162-173.
[17] Luo J, Lu K, Zhong Y, et al.2021. Cashmere and wool identification based on convolutional neural network[J]. Journal of Engineered Fibers and Fabrics, 16: 155892502110050.
[18] McGregor B A, Schlink A C.2014. Feltability of cashmere and other rare animal fibres and the effects of nutrition and blending with wool on cashmere feltability[J]. The Journal of The Textile Institute, 105(9): 927-937.
[19] O'Callaghan T F, Ross R P, Stanton C, et al.2016. The gut microbiome as a virtual endocrine organ with implications for farm and domestic animal endocrinology[J]. Domestic Animal Endocrinology, 56: S44-S55.
[20] Pan J, Feng R, Hu Q, et al.2020. TaqMan real-time quantitative PCR for identification of antlers in tradition Chinese medicine[J]. Mitochondrial DNA Part A, 31(5): 173-177.
[21] Qin X H, Liang H E, Su X Z, et al.2018. Study on properties of decolorizing yakwool fiber and spun yarn[J]. International Journal of Clothing Science and Technology, 31(1): 90-102.
[22] Schmidt A C, Jensen A A, Clausen A U, et al.2004. A comparative life cycle assessment of building insulation products made of stone wool, paper wool and flax[J]. International Journal of Life Cycle Assessment, 9(2): 122.
[23] Sofi A H, Wani M S, Ahmad S R, et al.2021. Comparative study on the quality characteristics of handmade and machine made pashmina shawls[J]. Journal of Natural Fibers, 1: 1-9.
[24] Subramanian S, Karthik T, Vijayaraaghavan N N.2005. Single nucleotide polymorphism for animal fibre identification[J]. Journal of Biotechnology, 116(2): 153-158.
[25] Taleb M A, Mowafi S, Vineis C, et al.2020. Effect of alkali metals and alkaline earth metals hydroxides on the structure of wool fibers[J]. Journal of Natural Fibers, 2020: 1846659.
[26] Tang M, Zhang W, Hui Z, et al.2014. A real-time PCR method for quantifying mixed cashmere and wool based on hair mitochondrial DNA[J]. Textile Research Journal, 84(15): 1612-1621.
[27] Xing W, Deng N, Xin B, et al.2019. An image-based method for the automatic recognition of cashmere and wool fibers[J]. Measurement, 141: 102-112.
[28] Zoccola M, Lu N, Mossotti R, et al.2013. Identification of wool, cashmere, yak, and angora rabbit fibers and quantitative determination of wool and cashmere in blend: A near infrared spectroscopy study[J]. Fibers and Polymers, 14(8): 1283-1289. |
|
|
|
