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| Antagonistic Activity and Related Biocontrol Factors Detection of Bacillus subtilis BS193 on Phytophthora capsici |
| WANG Rong-Bo1,2, CHEN Shu-Zun1,2, XIAO Xiao-Lu1,2, LI Ben-Jin1,2, LIU Pei-Qing1,2, SHI Ming-Yue1,2, CHEN Qing-He1,*, WENG Qi-Yong1,2,* |
1 Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2 Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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Abstract Pepper blight is a devastating soil-borne disease caused by Phytophthora capsici, which occurs generally in pepper (Capsicum annuum) growing areas worldwide, and causes huge economic loss. The antagonistic activity of Bacillus subtilis BS193 was assessed by dual culture test on V8 plates using 6 plant pathogenic oomycetes. The results showed that BS193 greatly inhibited the targeted pathogens with more than 50% inhibition, and showed the best inhibitory effect on Ph. capsici with the rate of 66.7%. When the BS193 cultural filtrates and Ph. capsici were inoculated simultaneously on pepper leaves, 63.50% disease suppression was observed. In pepper seedling inoculation test, the BS193 strain with concentration of 1×108 cfu/mL at 3 d post inoculation of Ph. capsici showed 89.52% pepper blight disease reduction. In addition, BS193 displayed the better potential in promoting plant growth in greenhouse environment. The height, root length, fresh weight and dry weight of pepper seedlings increased by 50.04%, 48.16%, 73.27% and 48.83%, respectively, after 30 d of root irrigation treatment with 20 mL (1×108 cfu/mL) fermentation broth of BS193 strain. The fermentation filtrate of BS193 could inhibit the mycelium growth and lead to a significant increase in mycelial branching, deformity and shrinkage. Meanwhile, 40% fermentation filtrate could significantly inhibit sporangia production, zoospore release and cyst germination with inhibition rate of more than 90%. Further, BS193 were evaluated for biocontrol traits by solid medium and transparent circle detection, and found that BS193 could form the biofilm and exhibited production of protease and cellulase. Overall, the results indicated that B. subtilis BS193 has significant inhibitory activity against Phytophthora pathogens, and the excellent effect of promoting plant growth, which can be considered as a candidate with great development potential for biological control of pepper blight.
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Received: 12 July 2021
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Corresponding Authors:
*wengqy@faas.cn; qhchen@hainanu.edu.cn
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