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| Over-expression of GbRac1 Gene Enhances the Tolerance to Phytophthora Root and Stem Rot in Soybean (Glycine max) |
| WANG Zhong-Wei1,2, ZHANG Yuan-Yu2, LIU Dong-Bo1,2, NIU Lu2, YANG Jing2, YANG Xiang-Dong2, ZHONG Xiao-Fang2,* |
1 School of Life Science, Jilin Normal University, Siping 136000, China;
2 Agricultural Biotechnology Institute/Jilin Provincial Key Laboratory of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China |
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Abstract Phytophthora root and stem rot (PRR) represents one of the destructive diseases in soybean (Glycine max) production, generally causing 10%~50% yield loss every year. Transgenic biotechnology provides an effective strategy for preventing and controlling the occurrence of PRR. The small G protein, known as the 'molecular switch', is not only involved in the process of regulating the adaptation of plants to external environmental stimuli during plant growth and development, but also participates in the establishment of plant defense responses. In this study, Gossypium barbadense Rac family small G protein 1 gene (GbRac1) was introduced into the soybean cultivar 'Williams 82'. Integration and expression of the foreign gene in transgenic soybean plants were confirmed by Southern blot and reverse transcription-PCR (RT-PCR) analysis. The PRR tolerance of the T3 and T4 generations of transgenic soybean lines was evaluated using a hypocotyl inoculation method. At 7 d after inoculation with Phytophthora sojae race 1, typical PRR symptoms such as wilting leaves and plant death were observed in the non-transformed plants. In contrast, most of transgenic plants showed no visible symptoms and developed normally with the survival rate of 70.68%~90.63%, higher than that of the control plants (40.74%~50.85%). However, no significant differences in agronomic traits were observed between the transgenic and non-transformed plants in the field conditions when no P. sojae inoculation involved. Taken together, these results demonstrated that over-expression of GbRac1 significantly enhanced the tolerance of the transgenic soybean to PRR without negative consequences on the agronomic performance. This results also pave the way for broadening disease resistance in soybean and development of the PRR-resistant soybean cultivars.
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Received: 02 June 2021
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Corresponding Authors:
*xfzhong649@163.com
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