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| Identification of OFP Gene Family and Their Expression Analysis Under Low-temperature Stress in Wheat (Triticum aestivum) |
| XU Ke1, WANG Wei-Wei1,2, WU Ning-Jing1, ZHANG Shu-Hua1, ZHAO Yong1,*, YANG Xue-Ju1,* |
1 College of Agronomy/State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, China;
2 Cangzhou Academy of Agriculture and Forestry Sciences, Cangzhou 061001, China |
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Abstract Ovate family proteins (OFPs) is a plant-specific transcription factor gene family, which plays an important role in low temperature stress response. In order to explore the OFP gene family members in wheat (Triticum aestivum), as well as the expression characteristics under cold stress, in this study, the members of the OFP family were identified and bioinformatics analysis was carried out in the whole genome of wheat, the expression pattern of TaOFPs in different tissues under low temperature stress were analyzed based on RNA-seq. The results showed that a total of 100 TaOFPs were identified, which were evenly distributed on A, B and D subgenomes. Wheat, rice (Oryza sativa) and Arabidopsis OFPs were divided into 6 subgroups based evolutionary analysis, and the OFP genes in gramineae were more conservative during evolution. The results of promoter elements analysis indicated that a large number of hormone related and abiotic stress response cis-acting elements, such as MeJA responsive elements, ABA responsive elements, low temperature responsive elements and drought responsive elements existed in promoter sequences of TaOFPs. The expression analysis revealed the tissue and stage specific expression patterns of TaOFPs. Under freezing stress, 25 TaOFPs were differentially expressed in cold acclimation and freezing specificity, TaOFP4-1D, TaOFP10-2B, TaOFP27-4D, TaOFP31-5A and TaOFP33-5D showed opposite expressed pattern in cold acclimation and freezing and un-cold acclimation and freezing treatments, suggested that these genes may involved in regulation of cold resistance of wheat. This study provides reference for further exploring the function of TaOFP family members and analyzing its regulatory mechanism in low-temperature stress.
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Received: 04 November 2020
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Corresponding Authors:
* shmyxj@126.com; zhaoyong_0423@163.com
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[1] 常缨, 张微, 李宏玲, 等. 2015. 转录因子AtOFP4影响拟南芥表皮细胞的伸长[J]. 东北农业大学学报, 46(03): 50-58, 73.
(Chang Y, Zhang W, Li H L, et al.2015. Effect of transcription factor AtOFP4 on epidermal cells elongation in Arabidopsis thaliana[J]. Journal of Northeast Agricultural University, 46(03): 50-58, 73.)
[2] 郭昱, 李娜, 李珊, 等. 2020. 谷子OFP基因家族的鉴定及表达分析[J]. 山西农业科学, 48(04): 467-476.
(Guo Y, Li N, Li S, et al.2020. Identification and expression analysis of OFP gene family in Setavia italica[J]. Journal of Shanxi Agricultural Science, 48(4): 467-476.)
[3] 黄笑非, 屈彤彤, 丁洋, 等. 2020. 赤霉素缺失型番茄果实抗冷性变化[J]. 食品与发酵工业, 46(13): 99-106.
(Huang X F, Qu T T, Ding Y, et al.2020. Chilling resistance of gibberellin-deficiency tomato fruits[J]. Food and Fermentation Industries, 46(13): 99-106.)
[4] 梁红凯, 陈辉龙, 杨溥原, 等. 2020. 高粱OFP转录因子家族生物信息学及盐胁迫下的表达分析[J]. 河北农业大学学报, 43(03): 1-8.
(Liang H K, Chen H L, Yang B Y, et al.2020. Bioinformatics analysis of OFP transcription factor family in Sorghum bicolor and expression analysis under salt stress[J]. Journal of Hebei Agricultural University, 43(3): 1-8.)
[5] 梁宜策, 薛理靠, 张军峰, 等. 2003. 小麦冻害调查初报[J]. 陕西农业科学, (5): 38-41.
(Liang Y C, Xue L K, Zhang J F, et al. 2003. Investigation on freezing injury of wheat [J], Shaanxi Journal of Agricultural Sciences, (5): 38-41.)
[6] 刘守银, 唐尧, 韩佳良, 等. 2020. 卵型家族蛋白转录因子AtOFP8的抗旱性功能分析[J]. 华北农学报, 35(05): 98-106.
(Liu S Y, Tang Y, Han J L, et al.2020. Drought resistance analysis of ovate family proteins transcription factor AtOFP8[J]. Acta Agriculturae Boreali-Sinica, 35(05): 98-106.)
[7] 王静雅, 刘文娟, 吕祎馨, 等. 2020. 油桐OFP基因家族的全基因组鉴定及低温响应分析[J]. 植物生理学报, 56(05): 949-960.
(Wang Y J, Liu W J, Lu W X, et al.2020. Genome-wide identification and low temperature response analysis of OFP gene family of in tung tree (Vernicia fordii)[J]. Plant Physiology Journal, 56(05): 949-960.)
[8] 许瑞瑞, 李睿, 王小非, 等. 2018. 苹果OFP基因家族的全基因组鉴定与非生物逆境表达分析[J].中国农业科学, 51(10): 1948-1959.
(Xu R R, Li R, Wang X F, et al.2018. Identification and expression analysis under abiotic stresses of OFP gene family in apple[J]. Scientia Agricultura Sinica, 51(10): 1948-1959.)
[9] 于慧. 2015. 水稻OsOFP转录因子家族基因克隆与功能分析[D]. 博士学位论文, 吉林大学, 导师: 林辰涛, pp. 107.
(Yu H.2015. The genes cloning and functional analysis of OsOFP transcription factor family in rice (Oryza sativa L.)[D]. Thesis of M.S., Jilin University, Supervisor: Lin C T, pp. 107.)
[10] 袁月, 张亚光, 高世敏, 等. 2016. 葡萄OVATE基因家族生物信息学及表达[J]. 中国农业科学, 49(19): 3786-3797.
(Yuan Y, Zhang Y G, Gao S M, et al.2016, Bioinformatics and expression of OVATE gene family in grape[J]. Chinese Agricultural Science, 49(19): 3786-3797.)
[11] 张彦琴, 常建忠, 董春林, 等. 2020. 玉米OFP基因家族全基因组鉴定及表达分析[J]. 分子植物育种, 18(20): 6595-6604.
(Zhang Y Q, Chang J Z, Dong C L, et al.2020. Genome-wide identification and expression analysis of OFP gene family in Zea mays[J]. Molecular Plant Breeding, 18(20): 6595-6604.)
[12] Chen C J, Chen H, Zhang Y, et al.2020. TBtools: An integrative toolkit developed for interactive analyses of big biological data[J]. Molecular Plant, S1674-2052(20): 30187-30188.
[13] Ding B, Hu C, Feng X, et al.2020. Systematic analysis of the OFP genes in six Rosaceae genomes and their roles in stress response in Chinese pear (Pyrus bretschneideri)[J]. Physiolgy and Moleculat Biology of Plants. 26(10):2085-2094.
[14] He Z, Zhang H, Gao S, et al.2016. Evolview v2: An online visualization and management tool for customized and annotated phylogenetic trees[J]. Nucleic Acids Research, 44(1): 236-241.
[15] Krzywinski M, Schein J, Birol I, et al.2009. Circos: An information aesthetic for comparative genomics[J]. Genome Research, 19(9): 1639-1645.
[16] Kumar S, Stecher G, Tamura K.2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 33(7): 1870-1874.
[17] Lescot M L, Dehais P, Thijs G, et al.2002. PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Research, 30(1): 325-327.
[18] Li E Y, Wang S C, Liu Y Y, et al.2011. OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana[J]. The Plant Journal, 67(2): 328-341.
[19] Li H F, Dong Q L, Zhu X P, et al.2019. Genome-wide identification, expression, and interaction analysis for ovate family proteins in peach[J]. Molecular Biology Reports, 46(4): 3755-3764.
[20] Liu D, Sun W, Yuan Y W, et al.2014. Phylogenetic analyses provide the first insights into the evolution of OVATE family proteins in land plants[J]. Annals of Botany, 113(7): 1219-1233.
[21] Liu J H, Zhang J, Hu W, et al.2015. Banana ovate family protein MaOFP1 and MADS-Box protein MuMADS1 antagonistically regulated banana fruit ripening[J]. PLOS ONE, 10(4): e0123870.
[22] Ma Y M, Yang C, He Y, et al.2017. Rice OVATE family protein 6 regulates plant development and confers resistance to drought and cold stresses[J]. Journal of Experimental Botany, 68(17): 4885-4898.
[23] Wang S C, Chang Y, Guo J J, et al.2011. Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development[J]. PLOS ONE, 6(8): e23896.
[24] Wang S, Chang Y, Guo J, et al.2007. Arabidopsis Ovate family protein 1 is a transcriptional repressor that suppresses cell elongation[J]. Plant Journal, 50(5): 858-872.
[25] Wang Y, Tang H, Debarry J D, et al.2012. MCScanX: A toolkit for detection and evolutionary analysis of gene synteny and collinearity[J]. Nucleic Acids Research, 40: e49.
[26] Winfield M O, Lu C, Wilson I D, et al.2010. Plant responses to cold: Transcriptome analysis of wheat[J]. Plant Biotechnology Journal, 8(7): 749-771.
[27] Xiao Y H, Liu D P, Zhang G X, et al.2017. Brassinosteroids regulate OFP 1, a DLT interacting protein, to modulate plant architecture and grain morphology in rice[J]. Frontiers in Plant Science, 8: 1698.
[28] Yang C, Ma Y M, He Y, et al.2018. OsOFP19 modulates plant architecture by integrating the cell division pattern and brassinosteroid signaling[J]. Plant Journal, 93(3): 489-501.
[29] Yang C, Shen W J, He Y, et al.2016. OVATE family protein 8 positively mediates brassinosteroid signaling through interacting with the GSK3-like kinase in rice[J]. PLOS Geneteic, 12(6): e1006118.
[30] Yu H, Jiang W Z, Liu Q, et al.2015. Expression pattern and subcellular localization of the ovate protein family in rice[J]. PLOS ONE, 10(3): e0118966.
[31] Zhao D S, Li Q F, Zhang C Q, et al.2018. GS9 acts as a transcriptional activator to regulate rice grain shape and appearance quality[J]. Nature Communication, 9(1): 1240.
[32] Zhao Y, Zhou M, Xu K, et al.2019. Integrated transcriptomics and metabolomics analyses provide insights into cold stress response in wheat[J]. The Crop Journal, 6(1): 857-866.
[33] Zhou S, Cheng X, Li F, et al.2019a. Overexpression of SlOFP20 in tomato affects plant growth, chlorophyll accumulation, and leaf senescence[J]. Frontier in Plant Science, 10: 1510.
[34] Zhou S G, Hu Z L, Li F F, et al.2019b. Overexpression of SlOFP20 affects floral organ and pollen development[J]. Horticulture Research, 6: 125. |
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