Genome-wide Identification and Expression Analysis of PIN Gene Family in Dendrobium catenatum
WANG Hao1,2, JIANG Wei-Wei1,2, GUO Ying-Chun1,2, SI Jin-Ping1,2,*, CHEN Dong-Hong1,2,*
1 State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; 2 National Innovation Alliance of Dendrobium catenatum Industry/Engineering Technology Research Center of Dendrobium catenatum of National Forestry and Grassland Administration, Hangzhou 311300, China
Abstract Auxin export carrier PIN-FORMED (PIN) family members mainly mediate auxin polar transport and asymmetric distribution. To better understand Dendrobium catenatum PIN (DcPIN) gene family, the present study performed genome-wide identification of DcPIN members, and further analyzed their phylogenetic relationships, gene structure, protein conserved motifs and domain composition, promoter cis-acting element, and expression profiles of both tissues and stress responses by means of bioinformatics and transcriptome data. The results showed that D. catenatum contained 21 DcPIN members that were divided into 7 clades. Among them, PIN9 clade was specific to monocot and contained high number of DcPIN9 genes in D. catenatum, which might be strongly relevant to its unique lifestyle and living environment. The upstream DcPIN promoters were rich in cis-regulatory elements related to hormones, light, development, and stress response. Transcriptome data showed the expression of DcPIN genes in different tissues could be totally divided into specific expression and constitutive expression. Transcriptome and qRT-PCR analysis under different treatments showed DcPIN1a/9a/9d/9g were in response to cold, DcPIN9a/9f/9h in response to drought, DcPIN3a/9a/9d/9h in response to JA, and DcPIN1c/3a/9a in response to the infection of Southern Blight pathogen Sclerotium delphinii. This result provides a theoretical basis for further investigating the function of D. catenatum PIN genes in the growth and development and environmental adaptation.
WANG Hao,JIANG Wei-Wei,GUO Ying-Chun, et al. Genome-wide Identification and Expression Analysis of PIN Gene Family in Dendrobium catenatum[J]. 农业生物技术学报, 2021, 29(9): 1649-1664.
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