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| Proteomic Analysis of Skin Tissue in IGF-I Transgenic Fine Wool Sheep (Ovis aries) Based on DIA Technology |
| ZHANG Yi-Yuan, GUO Yan-Hua, TANG Hong, GAO Lei, WANG Xin-Hua, ZHOU Ping, WANG Li-Min* |
| Research Institute of Animal Husbandry and Veterinary Medicine, Xinjiang Academy of Agricultural and Reclamation Sciences/State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Shihezi 832000, China |
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Abstract The wool yield of transgenic Chinese Merion fine wool sheep (Ovis aries) with insulin-like growth factor I (IGF-I) gene was significantly increased. In order to find out the change of skin protein expression related to wool production, data independent acquisition (DIA) proteome sequencing technology and bioinformatics analysis were used to analyze the total skin protein of transgenic sheep and wild-type sheep, which aimed to find specific proteins related to wool growth characteristics. Firstly, DIA proteomics method and liquid chromatography tandem mass spectrometry (LC-MS/MS) were used to sequence the tissues of IGF-I transgenic sheep and wild type sheep. The sequencing data were normalized, differentially expressed proteins were analyzed, Gene Ontology (GO) and KEGG enrichment analysis and candidate protein screening were carried out. The results showed that 2 914 proteins were obtained, including 930 differentially expressed proteins. Compared with wild type, 374 proteins were up-regulated and 556 proteins were down-regulated, and 18 differentially expressed proteins were related to wool traits. GO analysis showed that there were 24, 14 and 18 annotations to biological process, molecular function and cell component, respectively; KEGG pathway analysis showed that the differentially expression proteins were involved in 42 signaling pathways, including 8 signaling pathways such as serine/threonine kinase, secretory glycoprotein, gonadotropin releasing hormone, tumor suppressor protein, transforming growth factor-β, which was related to wool growth and development. This study is helpful to reveal the relationship between differential protein and wool traits and provides a reference for exploring the molecular mechanism of wool traits and molecular marker breeding.
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Received: 10 August 2020
Published: 01 May 2021
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Corresponding Authors:
*wanglm1980@126.com
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