<em>ERBB4</em>、<em>NRG1</em>基因真核表达载体构建及其在番鸭神经细胞中的表达

doi:10.3969/j.issn.1674-7968.2021.05.012

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Abstract Receptor tyrosine protein kinase 4 (ERBB4) and neuregulin 1 (NRG1) genes play an important role in the nervous system, the expression of ERBB4 gene in aggressive Cairna moschata was higher than that in normal group. In order to further explore the ERBB4 and NRG1 genes expression in nerve cells of Cairna moschata, pEGFP-C1-ERBB4 and pDsRED-C1-NRG1 eukaryotic expression vectors were built and transfected to nerve cells of Cairna moschata, then the expression levels of ERBB4 and NRG1 genes and the downstream Src homolog and collagen homolog (Shc) protein and growth factor receptor bound protein 2 (Grb2) in the NRG1-ERBB4 signaling pathway were detected by qRT-PCR. The results showed that the expression of ERBB4 and NRG1 in the transfected pEGFP-C1-ERBB4 and pDsRED-C1-NRG1 cells was significantly higher than those of pEGFP-C1 and pDsRED-C1 empty vector cells (P<0.01), indicating that pEGFP-C1-ERBB4 and pDsRED-C1-NRG1 were successfully overexpressed in nerve cells of Cairna moschata; The expression of NRG1 in transfected pEGFP-C1-ERBB4 cells was significantly higher than that in transfected pEGFP-C1 empty vector cells (P<0.01), and the expression of ERBB4 in transfected pDsRED-C1-NRG1 cells was significantly higher than that in transfected pEGFP-C1 empty vector cells (P<0.01), it showed that the overexpression of ERBB4 and NRG1 both the were up-regulated by each other; Compared with the transfected pEGFP-C1 and pDsRED-C1 empty vector and the blank experimental group, the expression of Shc and Grb2 in transfected pEGFP-C1-ERBB4 and pDsRED-C1-NRG1 cells were significantly up-regulated (P<0.01). The above results indicated that the expression up-regulated of ERBB4 or NRG1 significantly increased the expression of the other one, and the downstream genes Shc and Grb2 of NRG1-ERBB4 signaling pathway were increased in Cairna moschata nerve cells. The results of this study provide a basis for further exploring the mechanism of ERBB4 and NRG1 genes in the aggressive behavior of Cairna moschata.

Key words： Muscovy duck ERBB4 gene NRG1 gene Expression Aggressive behavior

Received: 02 July 2020 Published: 01 May 2021

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Corresponding Authors: *shenglinyang@126.com

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	LUO Lin-Li
	YANG Sheng-Lin
	ZHOU Xuan
	ZHANG Yun
	YANG Shi-Hao
	ZHU Yong-Cai

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LUO Lin-Li,YANG Sheng-Lin,ZHOU Xuan, et al. Construction of Eukaryotic Expression Vectors of ERBB4 and NRG1 Genes and Its Expression in Cairna moschata Nerve Cells[J]. 农业生物技术学报, 2021, 29(5): 952-962.

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http://journal05.magtech.org.cn/Jwk_ny/EN/10.3969/j.issn.1674-7968.2021.05.012 OR http://journal05.magtech.org.cn/Jwk_ny/EN/Y2021/V29/I5/952

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