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| Effects of Overexpression of St536 Gene on Cellulose Accumulation in Nicotiana tabacum |
| SA Shi-Juan1, YIN Qian1, WU Han-Yu1, XI Yun-Feng1, ZHENG Rui1,2, YAO Xin-Ling1,2,* |
1 Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Ningxia University, Yinchuan 750021, China;
2 Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resource in the Western China, Ningxia University, Yinchuan 750021, China |
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Abstract Mitochondrial membrane-binding proteins (MMBPs) encoded by nucleus genes involve in cellulose accumulation. However, more MMBPs need to be identified and characterized to disclose its role in cellulose accumulation metabolism. In this study, St536 (ID: PGSC0003DMG400012536), which was screened from the previous transcriptome analysis and encoding membrane-binding protein, was cloned in potato (Solanum tuberosum). Sequence analysis showed that the ORF of St536 gene was 648 bp, encoded 215 amino acid. Subcellular localization showed that the protein encoded by St536 gene was detected in the mitochondrial membrane. St536 overexpression vector was constructed and transformed into tobacco (Nicotiana tabacum) to obtain St536 overexpression lines of tobacco. Mophological assay and physiological analysis on the St536 overexpression lines showed that cellulose accumulation and the ratio of stomatal and guard cell relative vertical length (GL/SL) in the overexpression lines reduced 20% and 17%, respectively, comparing with wild type (WT). Stomatal conductance and photosynthetic rates in the overexpression lines were 2.4 and 2.5 times of the wild type, respectively. The result indicates that St536 overexpression decreased cellulose accumulation, lead to stomatal open and close easier, and enhanced stomatal conductance and photosynthesis rate. The study confirmed that St536 was involved in leaf cellulose accumulation, and provides basic data for further revealing the mechanism of cellulose accumulation.
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Received: 15 September 2020
Published: 01 May 2021
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Corresponding Authors:
*chinanoahl@163.com
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[1] Cvetkovska M, Dahal K, Alber N A, et al.2014. Knockdown of mitochondrial alternative oxidase induces the 'stress state' of signaling molecule pools in Nicotiana tabacum, with implications for stomatal function[J]. New Phytologist, 203(2): 449-461.
[2] Dahal K, Martyn G D, Alber N A, et al.2017. Coordinated regulation of photosynthetic and respiratory components is necessary to maintain chloroplast energy balance in varied growth conditions[J]. Journal of Experiment Botany, 68(3): 657-671.
[3] Djebbar R, Rzigui T, Pétriacq P, et al.2012. Respiratory complex I deficiency induces drought tolerance by impacting leaf stomatal and hydraulic conductances[J]. Planta, 235(3): 603-614.
[4] Doheny-Adams T, Hunt L, Franks P J, et al.2012. Genetic manipulation of stomatal density influences stomatal size, plant growth and tolerance to restricted water supply across a growth carbon dioxide gradient[J]. Philosophical Transactions-Royal Society. Biological Sciences, 367(1588): 547-555.
[5] Emanuelsson O, Nielsen H, Brunak S, et al.2000. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence[J]. Journal of Molecular Biology, 300(4): 1005-1016.
[6] Fanourakis D, Bouranis D, Giday H, et al.2016. Improving stomatal functioning at elevated growth air humidity: A review[J]. Journal Plant Physiology, 207: 51-60.
[7] Fraley R T, Rogers S G, Horsch R B,1986. Genetic transformation in higher plants[J]. Critical Reviews in Plant Sciences, 4(1): 1-46.
[8] Fromm S, Senkler J, Eubel H, et al.2016. Life without complex I: Proteome analyses of an Arabidopsis mutant lacking the mitochondrial NADH dehydrogenase complex[J]. Journal Experiment Botany, 67(10): 3079-3093.
[9] Hu Z, Vanderhaeghen R, Cools T, et al.2016. Mitochondrial defects confer tolerance against cellulose deficiency[J]. Plant Cell, 28(9): 2276-2290.
[10] Kardiman R, Ræbild A.2017. Relationship between stomatal density, size and speed of opening in Sumatran rainforest species[J]. Tree Physiology, 38(5): 696-705.
[11] Kleine T, Leister D.2016. Retrograde signaling: Organelles go networking[J]. Biochimica et Biophysica Acta, 1857(8): 1313-1325.
[12] Laitz A V, Acencio M L, Budzinski I G, et al.2015. Transcriptome response signatures associated with the overexpression of a mitochondrial uncoupling protein (AtUCP1) in tobacco[J]. PLOS ONE, 10(6): e0130744.
[13] Li L, Kubiszewski-Jakubiak S, Radomiljac J, et al.2016. Characterization of a novel β-barrel protein (AtOM47) from the mitochondrial outer membrane of Arabidopsis thaliana[J]. Journal of Experiment Botany, 67(21): 6061-6075.
[14] Liu J F, Feng XJ, Cui XQ, et al.2012. Expression of E. coliglgB in potatoes generating tuber starches with various features. Molecular Plant Breeding, 2012: 57-62.
[15] Marom Z, Shtein I, Bar-On B.2017. Stomatal opening: The role of cell-wall mechanical anisotropy and its analytical relations to the bio-composite characteristics[J]. Front in Plant Science, 8: 2061.
[16] Ng S, Ivanova A, Duncan O, et al.2013. A membrane-bound NAC transcription factor, ANAC017, mediates mitochondrial retrograde signaling in Arabidopsis[J]. Plant Cell, 25(9): 3450-3471.
[17] Rao R S P, Salvato F, Thal B, et al.2017. The proteome of higher plant mitochondria[J]. Mitochondrion, 33: 22-37.
[18] Rakić T, Gajic G, Lazarevic M, et al.2015. Effects of different light intensities, CO2 concentrations, temperatures and drought stress on photosynthetic activity in two paleoendemic resurrection plant species Ramonda serbica and R. nathaliae[J]. Environmental And Experimental Botany, 109: 63-72.
[19] Rui Y, Yi H, Kandemir B, et al.2016. Integrating cell biology, image analysis, and computational mechanical modeling to analyze the contributions of cellulose and xyloglucan to stomatal function[J]. Plant Signal & Behavour,11(6): e1183086.
[20] Schwarzländer M, Fuchs P.2017. Plant mitochondrial membranes: adding structure and new functions to respiratory physiology[J]. Current Opinion in Plant Biology,40:147-157.
[21] Van Dingenen J, Blomme J, Gonzalez N, et al.2016. Plants grow with a little help from their organelle friends[J]. Journal of Experiment Botany, 67(22): 6267-6281.
[22] Woolfenden H C, Bourdais G, Kopischke M, et al.2017. A computational approach for inferring the cell wall properties that govern guard cell dynamics[J]. Plant Journal for Cell & Molecular Biology, 92(1): 5-18.
[23] Zhang Y, Su J, Duan S, et al.2011. A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related process[J]. Plant Methods, 7: 30. |
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