Screening of Human BLM Gene-related LncRNAs and Analysis of Its Expression in Different Prostate Cancer Cells
LUO Bin-Jie1,2,3, YANG Li-Hong1,2,3, LI Yong1,2,3, CHEN Ying-Lian1,3,5, ZHAO Jia-Fu1,2,3,4, XU Hou-Qiang1,2,3,4,5,*
1 Key Laboratory of Genetics, Breeding and Reproduction of Plateau and Mountain Animals, Ministry of Education, College of Life Sciences, Guizhou University Guiyang 550025, China; 2 Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, College of Life Sciences, Guizhou University, Guiyang 550025, China; 3 College of Life Sciences, Guizhou University, Guiyang 550025, China; 4 College of Animal Science, Guizhou University, Guiyang 550025, China; 5 School of Medicine, Guizhou University, Guiyang 550025, China
Abstract BLM helicase (bloom helicase, BLM) plays an important role in DNA replication, repair, recombination, and maintenance of telomere stability. When the BLM helicase gene is mutated, it is prone to cause BLM syndrome and even cancers. To explore the expression patterns of long coding RNAs related to the BLM helicase gene. In this study, miR-27b-3p, which was related to the regulation of BLM genes, though the self-developed progame SWChen2.3 to screen the LncRNAs associated with the BLM genes, and an analyzed the BLM-associated with the LncRNAs in different prostate cancer cell lines PC3、Lncap、22RV-1 and normal fibroblst cell line WPMY-1, candidate LncRNAs were cloned, and their secondary structure and ORF were predicted, and target sites related to BLM gene were analyzed. NONHSAT203515.1 (m130127_152334_00126_c100) (named BLNC203) and NONHSAT246735.1 (lnc-ZBTB201:1) (named BLNC246) were selected and employed as research objects. The qRT-PCR method was used to detect the expression of BLNC203 and BLNC246 in 3 different prostate cancer cells and a normal epithelial cell line. The qRT-PCR results showed that the 2 LncRNAs were expressed in different prostate cancer cells, the expressions of BLNC203 in PC3 and 22Rv-1 were significantly higher than that in normal cells (P<0.01), while the differences of Lncap expression were not significant among these cells (P>0.05). The expressions of BLNC246 in PC3 and Lncap cells were significantly lower than that in normal cells (P<0.01), and while the differences of 22RV-1 expression were also not significant among these 22RV-1 cells (P>0.05). BLNC203 had 3 ORFs, BLNC246 had 1 ORF. the minimum free energy of the secondary structure of BLNC203 was -239.10 kcal/mol, and the minimum free energy of BLNC246 was -85.30 kcal/mol. The results of this study will provide a theoretical basis for further research on the mechanism of LncRNAs/BLM on the proliferation of prostate cancer cells.
LUO Bin-Jie,YANG Li-Hong,LI Yong, et al. Screening of Human BLM Gene-related LncRNAs and Analysis of Its Expression in Different Prostate Cancer Cells[J]. 农业生物技术学报, 2021, 29(5): 924-932.
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