Abstract Disease-resistant transgenic papaya (Carica papaya) has made great contribution in solving the problem of Papaya ring spot virus (PRSV). At present, the main transgenic papaya lines are '55-1', 'GM YK' and 'Huanong No.1'. In order to supervise the disease-resistant transgenic papaya products in the market and protect consumers' right to know and right to choose, it is necessary to establish more efficient and accurate detection method for transgenic papaya. This study analyzed the major genetically modified (GM) papaya in the world and established a simple and efficient multiplex PCR detection method for specific sequences of the papaya endogenous reference gene Papain, screening marker gene NPTⅡ, event-specific sequences of GM papaya lines '55-1', 'GM YK' and 'Huanong No.1'. The test results suggested that the established multiplex PCR detection method had high specificity and sensitivity. Samples could be judged whether they were transgenic papaya and which type of transgenic papaya were contained by only once PCR assay and electrophoresis. This study provides technical support for the composition detection and identity verification of transgenic papaya.
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